1,25-二羟基维生素D3在HL-60细胞分化过程中转录调控肿瘤坏死因子mRNA。
1,25-Dihydroxyvitamin D3 transcriptionally regulates tumour necrosis factor mRNA during HL-60 cell differentiation.
作者信息
Steffen M, Cayre Y, Manogue K R, Moore M A
机构信息
Laboratory of Developmental Hematopoiesi, Memorial Sloan-Kettering Cancer Center, New York.
出版信息
Immunology. 1988 Jan;63(1):43-6.
We investigated TNF-alpha gene expression in HL-60 cells under the influence of 1,25(OH)2D3. Our results show that TNF-alpha gene expression is transcriptionally activated by 1,25(OH)2D3. Despite the increase of TNF-alpha mRNA, very little TNF protein is found in either supernatants or cell lysates of HL-60 cells cultured in the presence of the vitamin. Therefore 1,25(OH)2D3 is regulating the transcription of the TNF-alpha gene in HL-60 cells without affecting translation. Our data suggest that an additional signal is necessary under physiological conditions to control post-transcriptionally TNF-alpha protein synthesis and secretion.
我们研究了1,25(OH)₂D₃作用下HL-60细胞中肿瘤坏死因子-α（TNF-α）基因的表达。我们的结果表明，TNF-α基因表达受到1,25(OH)₂D₃的转录激活。尽管TNF-α mRNA有所增加，但在添加该维生素培养的HL-60细胞的上清液或细胞裂解物中均未检测到TNF蛋白。因此，1,25(OH)₂D₃在不影响翻译的情况下调控HL-60细胞中TNF-α基因的转录。我们的数据表明，在生理条件下需要额外的信号来控制TNF-α蛋白转录后的合成与分泌。
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