Pudar Dubravka, Puggioli Arianna, Balestrino Fabrizio, Sy Victoria, Carrieri Marco, Bellini Romeo, Petrić Dušan
Laboratory for Medical and Veterinary Entomology, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia.
Sanitary Entomology & Zoology Department, Centro Agricoltura Ambiente "G. Nicoli" - IAEA Collaborating Centre, Crevalcore, Bologna, Italy.
Heliyon. 2021 Jun 22;7(6):e07381. doi: 10.1016/j.heliyon.2021.e07381. eCollection 2021 Jun.
is currently the most widespread invasive mosquito species in the world. It has paramount medical importance since females are efficient vectors of important viruses affecting humans The development of alternative control strategies to complement control measures has become an imperative and involves the Sterile Insect Technique (SIT). Research to improve the productivity of mass-rearing, as well as the quality of mass-reared males is of essential importance for the success of SIT.
This study compared the influence of three differently sized cages for mass-rearing on wing length, adult survival and egg production during 20 generations of colonization. Plexiglas cages of 40x40x40 cm (C1), 100 × 20 × 100 cm (C2) and 100 × 65 × 100 cm (C3) were loaded with equal adult density, and sex ratio of 1:1. An open source image processing and analysis programme (ImageJ) was used for the wing measurement and egg counting.
In all tested cages, we identified two periods separated by the generation showing the minimum value of each considered parameter (wing length, adult survival and egg production). The wing length and adult survival passed through the phases of initial decrease to about intermediate colonization time, and increased afterwards. Fecundity was steady during the first period and increased in the second one. Cage C1 demonstrated not only the best values for all parameters but also the smallest decrease in the initial phase. Recovering of the caged mosquitoes in the second half of the study was higher in cages C1 and C2, than in C3.
C1 provided the least negative selection pressure on wing length, adult survival and egg production for reared . Anyhow, since maximising mosquito density by exploiting the minimum space is a priority in mosquito mass-rearing, C2 might be a better choice for better fitting the space of mass-rearing rooms.
是目前世界上分布最广的入侵性蚊虫物种。由于雌蚊是影响人类的重要病毒的高效传播媒介，它具有至关重要的医学意义。开发替代控制策略以补充控制措施已成为当务之急，这涉及到昆虫不育技术（SIT）。提高大规模饲养的生产率以及大规模饲养雄蚊的质量的研究对于昆虫不育技术的成功至关重要。
本研究比较了三种不同尺寸的笼子在20代定殖过程中对大规模饲养的蚊虫翅长、成虫存活和产卵的影响。将40×40×40厘米（C1）、100×20×100厘米（C2）和100×65×100厘米（C3）的有机玻璃笼子装入相等的成虫密度，雌雄比例为1:1。使用开源图像处理和分析程序（ImageJ）进行翅长测量和卵计数。
在所有测试笼子中，我们确定了两个时期，以显示每个考虑参数（翅长、成虫存活和产卵）最小值的世代为界。翅长和成虫存活经历了最初下降到大约中间定殖时间的阶段，然后增加。繁殖力在第一个时期稳定，在第二个时期增加。笼子C1不仅在所有参数上表现出最佳值，而且在初始阶段下降最小。在研究的后半段，笼子C1和C2中笼养蚊子的回收率高于笼子C3。
C1对饲养的蚊虫翅长、成虫存活和产卵提供的负选择压力最小。无论如何，由于在蚊虫大规模饲养中优先考虑利用最小空间使蚊虫密度最大化，C2可能是更适合大规模饲养室空间的更好选择。
