Maïga Hamidou, Mamai Wadaka, Bimbilé Somda Nanwintoum Séverin, Wallner Thomas, Poda Bèwadéyir Serge, Salvador-Herranz Gustavo, Argiles-Herrero Rafael, Yamada Hanano, Bouyer Jérémy
Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, P.O. Box 100 A-1400 Vienna, Austria.
Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, 01 BP 545 Bobo-Dioulasso, Burkina Faso.
Insects. 2020 Nov 13;11(11):801. doi: 10.3390/insects11110801.
Successful implementation of the sterile insect technique (SIT) against and relies on a continuous supply of sterile males. To meet this requirement, optimization of the mass-rearing techniques is needed. This study, therefore, aims to assess a new mass-rearing cage (MRC) in terms of egg production efficiency and egg hatch rate (quality). In addition, adult survival was evaluated based on a cage adult-index for . Moreover, the cage's suitability for use in mass egg production was compared to that of the FAO/IAEA reference cage. In rearing, the new MRC produced 1,112,110 eggs per cage following six blood meals, with minimum loss of eggs in the egging water. Furthermore, the adult index gave a good proxy of daily mortality rates in . In rearing, about 130,000 eggs per egg batch were collected both from the new and the reference MRC. These findings suggest that the new MRC prototype is efficient in terms of egg production and can be used for mass-rearing in SIT programs targeting as well as . The adult index was also positively validated for the detection of unusual mortality rates in mass-rearing facilities. Overall, the new MRC has shown several advantages; however, further improvements are necessary to minimize escapes during the egg collection processes.
成功实施针对[具体对象1]和[具体对象2]的昆虫不育技术（SIT）依赖于持续供应不育雄虫。为满足这一需求，需要优化大规模饲养技术。因此，本研究旨在从产卵效率和卵孵化率（质量）方面评估一种新型大规模饲养笼（MRC）。此外，基于[具体对象1]的笼内成虫指数评估成虫存活率。此外，还将该笼在大规模产卵中的适用性与粮农组织/国际原子能机构参考笼进行了比较。在[具体对象1]饲养中，新型MRC在六次血餐后每个笼子产出1,112,110枚卵，在产卵水中的卵损失最小。此外，成虫指数很好地代表了[具体对象1]的每日死亡率。在[具体对象2]饲养中，新型MRC和参考MRC每个卵批次均收集到约130,000枚卵。这些发现表明，新型MRC原型在产卵方面效率较高，可用于针对[具体对象1]和[具体对象2]的昆虫不育技术项目中的大规模饲养。成虫指数在检测[具体对象2]大规模饲养设施中的异常死亡率方面也得到了积极验证。总体而言，新型MRC已显示出若干优势；然而，有必要进一步改进以尽量减少在收集卵过程中的逃逸情况。
