Martin S A, Falkler W A, Vincent J W, Mackler B F, Suzuki J B
Department of Microbiology, Baltimore College of Dental Surgery, University of Maryland Dental School 21201.
J Periodontol. 1988 Jan;59(1):32-9. doi: 10.1902/jop.1988.59.1.32.
Immunoglobulins in sera and in supernatant fluids of explant cultures of diseased gingival tissues from 20 rapidly progressive and 20 adult periodontitis sites were tested by an ELISA assay for reactivity with typed strains of Eubacterium alactolyticum, E. brachy, E. limosum and E. nodatum. Immunoglobulins present in tissue culture fluids from both rapidly progressive and adult periodontitis samples reactive with E. brachy and E. nodatum were significantly greater (P less than 0.05) than those reactive with E. alactolyticum or E. limosum. The titers to E. brachy in tissue culture fluids from adult periodontitis were significantly greater (P less than 0.05) than those from rapidly progressive periodontitis; there was no difference in titers to the other three species. The only significant difference in serum titers was that sera from patients with rapidly progressive periodontitis had significantly greater reactivity to E. alactolyticum than did sera from adult periodontitis patients. These data indicate that immunoglobulins in the sera of rapidly progressive and adult periodontitis patients do not necessarily reflect the reactivity of localized immunoglobulins present in the diseased gingival tissue explant culture fluids from these patients.
采用酶联免疫吸附测定法（ELISA），检测了来自20个快速进展型牙周炎位点和20个成人牙周炎位点的患病牙龈组织外植体培养物的血清及上清液中的免疫球蛋白，以检测其与解乳糖优杆菌、短优杆菌、黏液优杆菌和瘤优杆菌分型菌株的反应性。快速进展型牙周炎样本和成人牙周炎样本的组织培养液中，与短优杆菌和瘤优杆菌反应的免疫球蛋白显著多于（P<0.05）与解乳糖优杆菌或黏液优杆菌反应的免疫球蛋白。成人牙周炎组织培养液中针对短优杆菌的滴度显著高于（P<0.05）快速进展型牙周炎组织培养液中的滴度；针对其他三种菌的滴度无差异。血清滴度的唯一显著差异在于，快速进展型牙周炎患者的血清对解乳糖优杆菌的反应性显著高于成人牙周炎患者的血清。这些数据表明，快速进展型牙周炎患者和成人牙周炎患者血清中的免疫球蛋白不一定能反映这些患者患病牙龈组织外植体培养液中局部免疫球蛋白的反应性。
