Vincent J W, Falkler W A, Heath J R
J Periodontol. 1984 Feb;55(2):93-7. doi: 10.1902/jop.1984.55.2.93.
Recent studies have demonstrated an association of Eubacterium sp. with the subgingival microflora of patients with chronic periodontitis. One species, Eubacterium brachy, was evaluated to determine the possible mechanisms by which this microorganism may contribute to this disease. An extracellular antigen was identified in the culture supernatant which reacted with antibodies in human sera. This antigen was isolated by methanol precipitation and purified by gel filtration. The purified extracellular antigen was reacted in vitro with 45CaCl2-labeled fetal rat bone in a bone resorptive bioassay. This antigen was shown to have a molecular weight of 170,000, to share a line of identity with a sonicated preparation of E. brachy whole cells and to result in increased 45CaCl2 release from fetal rat bones when cultures were exposed to the purified extracellular antigen at concentrations of 10 to 53 micrograms/ml.
近期研究表明，真杆菌属与慢性牙周炎患者的龈下微生物群有关联。对其中一种短真杆菌进行了评估，以确定这种微生物可能导致该疾病的潜在机制。在培养上清液中鉴定出一种细胞外抗原，它能与人血清中的抗体发生反应。该抗原通过甲醇沉淀法分离，并通过凝胶过滤法纯化。纯化后的细胞外抗原在体外骨吸收生物测定中与45CaCl2标记的胎鼠骨反应。结果显示，这种抗原的分子量为170,000，与短真杆菌全细胞的超声破碎制剂具有相同的抗原条带，当培养物暴露于浓度为10至53微克/毫升的纯化细胞外抗原时，会导致胎鼠骨中45CaCl2释放增加。
