Vincent J W, Falkler W A, Dalessandro N F, Miller R A, Heath J R
Infect Immun. 1985 Mar;47(3):592-7. doi: 10.1128/iai.47.3.592-597.1985.
Recent studies have demonstrated an association of Eubacterium sp. with the subgingival microflora of patients with chronic periodontitis. One species, Eubacterium brachy, was evaluated to determine the possible mechanisms by which this microorganism may contribute to these diseases. Of 167 sera evaluated by double diffusion in agar, 20.8% displayed reactivity with a sonicated preparation of E. brachy. An extracellular antigen was identified in the culture supernatant fluid which reacted with antibodies in human sera. This antigen was isolated by methanol precipitation and purified by gel filtration. When tested by an enzyme-linked immunosorbent assay, all sera displayed some reactivity. Lines of identity were not shared with other species of Eubacterium, but were shared with other clinical isolates of E. brachy. The reactive antibody was identified as immunoglobulin G by immunoelectrophoresis, verified by enzyme-linked immunosorbent assay, and found to be capable of complement fixation. The monosaccharides and amino acids of the extracellular antigen were identified by high-pressure liquid chromatography. This antigen was shown to have a molecular weight of 170,000 and to share a line of identity with the sonicated preparation of E. brachy. The possible role of the organism in the immunopathology of periodontal diseases is discussed.
最近的研究表明，真杆菌属与慢性牙周炎患者的龈下微生物群有关联。对其中一个物种——短真杆菌进行了评估，以确定这种微生物可能导致这些疾病的潜在机制。在通过琼脂双扩散法评估的167份血清中，20.8%与短真杆菌的超声破碎制剂发生反应。在培养上清液中鉴定出一种细胞外抗原，它与人血清中的抗体发生反应。该抗原通过甲醇沉淀分离，并通过凝胶过滤纯化。通过酶联免疫吸附测定法进行检测时，所有血清均表现出一定的反应性。其反应线与其他真杆菌属物种不相同，但与短真杆菌的其他临床分离株相同。通过免疫电泳将反应性抗体鉴定为免疫球蛋白G，经酶联免疫吸附测定法验证，并发现其能够固定补体。通过高压液相色谱法鉴定了细胞外抗原的单糖和氨基酸。该抗原的分子量为170,000，并且与短真杆菌的超声破碎制剂具有相同的反应线。文中讨论了该生物体在牙周疾病免疫病理学中的可能作用。
