Winkler J R, Matarese V, Hoover C I, Kramer R H, Murray P A
Department of Stomatology, School of Dentistry, University of California, San Francisco 94143-0515.
J Periodontol. 1988 Jan;59(1):40-5. doi: 10.1902/jop.1988.59.1.40.
In periodontal disease, the abilities of bacteria to adhere to and degrade in vivo basement membranes should be considered as two of the rate-limiting steps for the potential active or passive invasion of gingival connective tissues. To study these mechanisms in greater detail, we used the PF HR-9 basement-membrane-like matrix to establish an in vitro model of bacterial invasion and degradation. Three gram-negative anaerobic periodontopathic organisms, Bacteroides gingivalis, Fusobacterium nucleatum, and Actinobacillus actinomycetemcomitans, bound in considerably higher numbers to the HR-9 matrix than did 6 strains of gram-positive facultative organisms typically associated with periodontal health. In a further experiment with B. gingivalis, the organism rapidly degraded Type IV collagen, the major macromolecular component constituting the HR-9 matrix. Streptococcus mitis, the nonperiodontopathic bacterium tested, did not degrade this model matrix. This study provides evidence that B. gingivalis, a periodontopathic bacterium, is able to adhere to and degrade basement membranes, whereas nonperiodontopathic organisms appear not to share in these abilities.
在牙周疾病中，细菌黏附于体内基底膜并在其中降解的能力，应被视为牙龈结缔组织潜在主动或被动侵袭过程中的两个限速步骤。为了更详细地研究这些机制，我们使用PF HR - 9基底膜样基质建立了细菌侵袭和降解的体外模型。三种革兰氏阴性厌氧牙周病原菌，牙龈卟啉单胞菌、具核梭杆菌和伴放线聚集杆菌，与HR - 9基质的结合数量明显高于通常与牙周健康相关的6株革兰氏阳性兼性菌。在进一步用牙龈卟啉单胞菌进行的实验中，该菌能迅速降解构成HR - 9基质的主要大分子成分IV型胶原。所测试的非牙周病原菌缓症链球菌则不能降解这种模型基质。本研究提供了证据表明，牙周病原菌牙龈卟啉单胞菌能够黏附并降解基底膜，而非牙周病原菌似乎不具备这些能力。
