角蛋白酶基因在皮肤癣菌中的活性：对伊朗德黑兰临床和非临床样本中疣状毛癣菌和石膏样小孢子菌中角蛋白酶基因存在情况的研究。
Subtilisin Gene Activity in Dermatophytes: A study on the Presence of the Subtilisin Gene in Trichophyton verrucosum and Microsporum gypseum in Clinical and Nonclinical Samples in Tehran, Iran.
机构信息
Department of Pathobiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Mycology and Parasitology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Arch Razi Inst. 2021 Jul;76(2):253-259. doi: 10.22092/ari.2019.126417.1343. Epub 2021 Jul 1.
The keratinolytic activities of dermatophyte species are accompanied by the secretion of enzymes, such as serine proteases, which are coded by the Subtilisin (SUB) genes. This study aimed to determine the presence of the SUB genes in the clinical and nonclinical samples of Trichophyton verrucosum and Microsporum gypseum. Isolation was carried out by direct and laboratory examination. Following that, for the determination of the presence of the SUB gene, polymerase chain reaction with specific primers was conducted. The frequencies of the SUB gene were observed in almost 66% of the isolates. Statistical analysis showed a significant relationship between the presence of the SUB gene and the samples collected from human, animals, and soil (p ˂0.005). The current investigation has been the first study of the presence/absence of the SUB gene in the clinical and nonclinical isolates of T. verrucosum and M. gypseum in Iran which may be a new step to perform further studies.
角蛋白水解酶活性伴随着真菌分泌酶，如丝氨酸蛋白酶，这些酶由枯草杆菌蛋白酶（SUB）基因编码。本研究旨在确定临床和非临床样本中疣状毛癣菌和石膏样小孢子菌是否存在 SUB 基因。通过直接和实验室检查进行分离。随后，通过特异性引物聚合酶链反应确定 SUB 基因的存在。几乎 66%的分离株存在 SUB 基因。统计分析显示，SUB 基因的存在与从人、动物和土壤中采集的样本之间存在显著关系（p ˂0.005）。本研究首次在伊朗临床和非临床分离株中检测到 SUB 基因的存在/缺失，这可能是进一步研究的新步骤。
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