Unit of Microbiology, Department of Biomedical Sciences & Public Health, Polytechnic University of Marche Medical School, Ancona, Italy.
Unit of Microbiology, Department of Life & Environmental Sciences, Polytechnic University of Marche, Ancona, Italy.
Future Microbiol. 2021 Jul;16:703-711. doi: 10.2217/fmb-2020-0246. Epub 2021 Jul 5.
This study reports the characterization of carbapenem-resistant colonizing strains of . 650 stool samples were screened for carbapenem-resistant (CR-Kp). All strains were characterized for antibiotic susceptibility, typing features, main carbapenemases and extended-spectrum ß-lactamases. The carbapenemase transferability was assessed by interspecific conjugation. Eighteen CR-Kp were multidrug resistant, five were KPC producing. A predominance of ST307 isolates, constituting the predominant cluster by PFGE analysis, was identified (50% were KPC-2 producers). Conjugation data showed the co-transfer of KPC-2, TEM-1, OXA-1, CTX-M-15 in a single large pKPN3-like plasmid. Our data pointed out the diversity of colonizing strains compared with clinical ones. The predominance of ST307 strains suggested an increased spreading, even in our area, of this high-risk clone.
本研究报告了碳青霉烯类耐药定植株的特征。对 650 份粪便样本进行了碳青霉烯类耐药（CR-Kp）筛查。所有菌株均进行了抗生素敏感性、分型特征、主要碳青霉烯酶和超广谱β-内酰胺酶的特征分析。通过种间接合评估了碳青霉烯酶的可转移性。18 株 CR-Kp 为多药耐药，5 株产 KPC。通过 PFGE 分析发现，ST307 分离株占优势，构成主要聚类（50%为 KPC-2 产生者）。接合数据显示，KPC-2、TEM-1、OXA-1 和 CTX-M-15 可共同转移到单个大的 pKPN3 样质粒中。我们的数据表明，与临床菌株相比，定植株具有多样性。ST307 菌株的优势表明，即使在我们所在地区，这种高风险克隆的传播也在增加。
