Piccirilli Alessandra, Cherubini Sabrina, Azzini Anna Maria, Tacconelli Evelina, Lo Cascio Giuliana, Maccacaro Laura, Bazaj Alda, Naso Laura, Amicosante Gianfranco, Perilli Mariagrazia
Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Infectious Disease Section, Department of Diagnostic and Public Health, University of Verona, 37134 Verona, Italy.
Microorganisms. 2021 Sep 17;9(9):1985. doi: 10.3390/microorganisms9091985.
(KPN) is one of the widest spread bacteria in which combined resistance to several antimicrobial groups is frequent. The most common β-lactamases found in are class A carbapenemases, both chromosomal-encoded (i.e., NMCA, IMI-1) and plasmid-encoded (i.e., GES-enzymes, IMI-2), VIM, IMP, NDM, OXA-48, and extended-spectrum β-lactamases (ESBLs) such as CTX-M enzymes. In the present study, a total of 68 carbapenem-resistant KPN were collected from twelve long-term care facilities (LTCFs) in the Northern Italian region. The whole-genome sequencing (WGS) of each KPN strain was determined using a MiSeq Illumina sequencing platform and analysed by a bacterial analysis pipeline (BAP) tool. The WGS analysis showed the prevalence of ST307, ST512, and ST37 as major lineages diffused among the twelve LTCFs. The other lineages found were: ST11, ST16, ST35, ST253, ST273, ST321, ST416, ST1519, ST2623, and ST3227. The , , , , , , , , , , , , , and were the resistance genes widespread in most LTCFs. In this study, we demonstrated the spreading of thirteen KPN lineages among the LTCFs. Additionally, KPC carbapenemases are the most widespread β-lactamase.
肺炎克雷伯菌（KPN）是传播最广泛的细菌之一，对多种抗菌药物类别产生联合耐药的情况很常见。在肺炎克雷伯菌中发现的最常见β-内酰胺酶是A类碳青霉烯酶，包括染色体编码的（即NMC-A、IMI-1）和质粒编码的（即GES酶、IMI-2）、VIM、IMP、NDM、OXA-48以及超广谱β-内酰胺酶（ESBLs），如CTX-M酶。在本研究中，从意大利北部地区的12家长期护理机构（LTCFs）收集了总共68株耐碳青霉烯类肺炎克雷伯菌。使用MiSeq Illumina测序平台对每株肺炎克雷伯菌菌株进行全基因组测序（WGS），并通过细菌分析流程（BAP）工具进行分析。WGS分析显示，ST307、ST512和ST37作为主要菌系在12家LTCFs中广泛传播。发现的其他菌系有：ST11、ST16、ST35、ST253、ST273、ST321、ST416、ST1519、ST2623和ST3227。blaKPC、blaVIM、blaIMP、blaNDM、blaOXA-48、blaCTX-M-15、blaCTX-M-27、blaCTX-M-65、blaSHV、blaTEM、blaOXA-1、blaOXA-2和blaOXA-10是在大多数LTCFs中广泛存在的耐药基因。在本研究中，我们证明了13种肺炎克雷伯菌菌系在LTCFs中的传播。此外，KPC碳青霉烯酶是分布最广泛的β-内酰胺酶。
