研究论文：表达肺表面活性蛋白-A 和 D 以及 CD9 在 1 天和 30 天龄小马驹肺中的作用。

Research article expression of surfactant protein-A and D, and CD9 in lungs of 1 and 30 day old foals.

机构信息

Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.

Department of Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.

出版信息

BMC Vet Res. 2021 Jul 5;17(1):236. doi: 10.1186/s12917-021-02943-5.

DOI:10.1186/s12917-021-02943-5

PMID:34225699

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8256609/

Abstract

BACKGROUND

Respiratory diseases are a major cause of morbidity and mortality in the horses of all ages including foals. There is limited understanding of the expression of immune molecules such as tetraspanins and surfactant proteins (SP) and the regulation of the immune responses in the lungs of the foals. Therefore, the expression of CD9, SP-A and SP-D in foal lungs was examined.

RESULTS

Lungs from one day old (n = 6) and 30 days old (n = 5) foals were examined for the expression of CD9, SP-A, and SP-D with immunohistology and Western blots. Western blot data showed significant increase in the amount of CD9 protein (p = 0.0397) but not of SP-A and SP-D at 30 days of age compared to one day. Immunohistology detected CD9 in the alveolar septa and vascular endothelium but not the bronchiolar epithelium in the lungs of the foals in both age groups. SP-A and SP-D expression was localized throughout the alveolar septa including type II alveolar epithelial cells and the vascular endothelium of the lungs in all the foals. Compared to one day old foals, the expression of SP-A and SP-D appeared to be increased in the bronchiolar epithelium of 30 day old foals. Pulmonary intravascular macrophages were also positive for SP-A and SP-D in 30 days old foals and these cells are not developed in the day old foals.

CONCLUSIONS

This is the first data on the expression of CD9, SP-A and SP-D in the lungs of foals.

摘要

背景

呼吸道疾病是导致所有年龄段马匹（包括幼驹）发病和死亡的主要原因。人们对幼驹肺部中免疫分子（如四跨膜蛋白和表面活性剂蛋白）的表达和免疫反应的调节知之甚少。因此，本研究检测了幼驹肺部中 CD9、SP-A 和 SP-D 的表达。

结果

用免疫组织化学和 Western blot 检测了 1 日龄（n=6）和 30 日龄（n=5）幼驹肺部中 CD9、SP-A 和 SP-D 的表达。Western blot 数据显示，与 1 日龄相比，30 日龄幼驹肺部的 CD9 蛋白量显著增加（p=0.0397），但 SP-A 和 SP-D 蛋白量没有增加。免疫组织化学检测到 CD9 在肺泡隔和血管内皮中表达，但在两个年龄段的幼驹的支气管上皮中均未检测到。SP-A 和 SP-D 表达定位于所有幼驹肺部的肺泡隔，包括 II 型肺泡上皮细胞和血管内皮。与 1 日龄幼驹相比，30 日龄幼驹的支气管上皮中 SP-A 和 SP-D 的表达似乎增加。30 日龄幼驹的肺血管内巨噬细胞也对 SP-A 和 SP-D 呈阳性，而 1 日龄幼驹中没有这种细胞。