College of life sciences, Jiangxi Normal university, Nanchang, Jiangxi, China.
Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Ji'an, Jiangxi, China.
Environ Toxicol. 2021 Oct;36(10):2062-2072. doi: 10.1002/tox.23323. Epub 2021 Jul 6.
Quercetin is a flavonoid compound with a variety of biological properties that is widely distributed throughout the plant kingdom. Studies have found that quercetin has anti-inflammatory, antioxidant, and liver-protective effects, while thioacetamide (TAA) can cause inflammation and liver damage in zebrafish larvae. The purpose of this study was to evaluate whether quercetin can prevent TAA-induced inflammation and liver damage in zebrafish larvae and to investigate the molecular mechanisms involved. Zebrafish Tg transgenic lines were used as the experimental animals. Behavioral, oxidative stress level, proliferative antigen chromogenic antibody, and western blot analyses were carried out on zebrafish larvae in the control group and groups treated with TAA and 12 μM quercetin. The results indicated that quercetin promoted the development of zebrafish larvae damaged by TAA, exhibited antioxidant and anti-inflammatory properties, and promoted cell proliferation. Quercetin reduced the expression of p53 protein in zebrafish larvae injured by TAA, resulting in decreased levels of Bax and increased levels of Bcl-2. The findings suggested quercetin has antiapoptotic action. Quercetin reduced the expression of DKK1 and DKK2 genes related to the Wnt signaling pathway in zebrafish larvae damaged by TAA and increased the expression of Lef1 and wnt2bb. Quercetin may regulate the development of zebrafish larvae damaged by TAA through the Wnt signaling pathway. This study provides the scientific basis for the development and utilization of quercetin and the development of new related drugs.
槲皮素是一种具有多种生物学特性的类黄酮化合物，广泛分布于植物界。研究发现，槲皮素有抗炎、抗氧化和保肝作用，而硫代乙酰胺（TAA）可引起斑马鱼幼虫炎症和肝损伤。本研究旨在评价槲皮素是否能预防 TAA 诱导的斑马鱼幼虫炎症和肝损伤，并探讨其相关分子机制。以斑马鱼 Tg 转基因系为实验动物，对对照组和 TAA 及 12 μM 槲皮素处理组的斑马鱼幼虫进行行为学、氧化应激水平、增殖抗原显色抗体和 Western blot 分析。结果表明，槲皮素促进了 TAA 损伤的斑马鱼幼虫的发育，表现出抗氧化和抗炎特性，并促进了细胞增殖。槲皮素降低了 TAA 损伤的斑马鱼幼虫中 p53 蛋白的表达，导致 Bax 减少和 Bcl-2 增加。研究结果表明槲皮素有抗凋亡作用。槲皮素降低了 TAA 损伤的斑马鱼幼虫中与 Wnt 信号通路相关的 DKK1 和 DKK2 基因的表达，增加了 Lef1 和 wnt2bb 的表达。槲皮素可能通过 Wnt 信号通路调节 TAA 损伤的斑马鱼幼虫的发育。本研究为槲皮素的开发利用和相关新药的开发提供了科学依据。
