Vázquez-Atanacio María José, Bautista Mirandeli, González-Cortazar Manasés, Romero-Estrada Antonio, De la O-Arciniega Minarda, Castañeda-Ovando Araceli, Sosa-Gutiérrez Carolina G, Ojeda-Ramírez Deyanira
Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km 1, Ex-Hda. de Aquetzalpa, Tulancingo 43600, Hidalgo, Mexico.
Área Académica de Farmacia, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex Hacienda la Concepción s/n, San Agustín Tlaxiaca 42160, Hidalgo, Mexico.
Plants (Basel). 2022 Dec 10;11(24):3460. doi: 10.3390/plants11243460.
Acute kidney injury and impaired kidney function is associated with reduced survival and increased morbidity. is an edible plant endemic to Mexico used in Mexican traditional medicine. The aim of this study was to evaluate the nephroprotective effect of a hydroalcoholic extract (MeOH:water 70:30, ) from the aerial parts of (HEPr). Firstly, in vitro the antioxidant and anti-inflammatory activity of HEPr was determined; after the in vivo nephroprotective activity of HEPr was evaluated using a thioacetamide-induced injury model in rats. HEPr showed a slight effect on LPS-NO production in macrophages (15% INO at 40 µg/mL) and high antioxidant activity in the ferric reducing antioxidant power (FRAP) test, followed by the activity on DPPH and ABTS radicals test (69.04, 63.06 and 32.96% of inhibition, respectively). In addition, values of kidney injury biomarkers in urine (urobilinogen, hemoglobin, bilirubin, ketones, glucose, protein, pH, nitrites, leukocytes, specific gravity, and the microalbumin/creatinine) and serum (creatinine, urea, and urea nitrogen) of rats treated with HEPr were maintained in normal ranges. Finally, 5--caffeoylquinic, 4--caffeoylquinic and ferulic acids; as well as 3--quercetin glucoside and 3--kaempferol glucoside were identified by HPLC as major components of HEPr. In conclusion, constitutes a source of compounds for the treatment of acute kidney injury.
急性肾损伤和肾功能受损与生存率降低和发病率增加相关。[此处原文缺失植物名称]是墨西哥特有的一种可食用植物，用于墨西哥传统医学。本研究的目的是评估从[此处原文缺失植物名称]地上部分提取的水醇提取物（甲醇:水 70:30，[此处原文缺失提取条件相关内容]）（HEPr）的肾保护作用。首先，在体外测定了 HEPr 的抗氧化和抗炎活性；之后，在体内使用硫代乙酰胺诱导的大鼠损伤模型评估了 HEPr 的肾保护活性。HEPr 对巨噬细胞中 LPS-NO 的产生有轻微影响（40 µg/mL 时抑制率为 15%），在铁还原抗氧化能力（FRAP）试验中具有高抗氧化活性，其次是在 DPPH 和 ABTS 自由基试验中的活性（分别为 69.04%、63.06%和 32.96%的抑制率）。此外，用 HEPr 处理的大鼠尿液（尿胆原、血红蛋白、胆红素、酮体、葡萄糖、蛋白质、pH、亚硝酸盐、白细胞、比重以及微量白蛋白/肌酐）和血清（肌酐、尿素和尿素氮）中的肾损伤生物标志物值保持在正常范围内。最后，通过高效液相色谱法鉴定出 5 - 咖啡酰奎尼酸、4 - 咖啡酰奎尼酸和阿魏酸；以及 3 - 槲皮素葡萄糖苷和 3 - 山柰酚葡萄糖苷是 HEPr 的主要成分。总之，[此处原文缺失植物名称]构成了治疗急性肾损伤的化合物来源。
