Department of Biology and Function in Head and Neck, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Department of Otorhinolaryngology, Yokosuka Kyosai Hospital, Yokosuka, Japan.
Anticancer Res. 2021 Jul;41(7):3317-3326. doi: 10.21873/anticanres.15119.
BACKGROUND/AIM: We evaluated the impact of FosL1, a member of the activated protein-1 family, on the pathways leading to regional metastasis of head and neck squamous cell carcinoma (HNSCC).
We examined the influence of small interfering RNA (siRNA) and short heparin RNA (shRNA) mediated knockdown of FosL1 on cell migration, invasion, and proliferation in vitro as well as on regional metastasis in vivo. The prognostic significance of FosL1 was also analyzed using the Kaplan- Meier plotter using data from an HNSCC patient database.
Down-regulation of FosL1 inhibited cell migration, invasion, and proliferation in vitro, decreased the incidence of regional metastases, and prolonged the survival of mice in vivo. We also determined that HNSCC patients with higher expression levels of FosL1 had a significantly shorter survival time than those with low expression of FosL1.
FosL1 plays a crucial role in promoting cell migration, invasion, and proliferation in HNSCC.
背景/目的：我们评估了激活蛋白-1 家族成员 FosL1 对导致头颈部鳞状细胞癌（HNSCC）区域性转移的途径的影响。
我们研究了小干扰 RNA（siRNA）和短肝素 RNA（shRNA）介导的 FosL1 敲低对体外细胞迁移、侵袭和增殖以及体内区域性转移的影响。我们还使用 HNSCC 患者数据库中的数据，通过 Kaplan-Meier 绘图器分析了 FosL1 的预后意义。
下调 FosL1 抑制了体外细胞迁移、侵袭和增殖，降低了区域性转移的发生率，并延长了体内小鼠的存活时间。我们还确定，FosL1 表达水平较高的 HNSCC 患者的生存时间明显短于 FosL1 低表达的患者。
FosL1 在促进 HNSCC 中的细胞迁移、侵袭和增殖中起着关键作用。
