Wang Wei, Liu Wei, Xu Jing, Jin Hongze
Department of Neurosurgery, Changxing People's Hospital, Changxing, Zhejiang, China.
Front Genet. 2022 Aug 17;13:953580. doi: 10.3389/fgene.2022.953580. eCollection 2022.
To determine the relationship between glioma and muscle aging and to predict prognosis by screening for co-expressed genes, this study examined the relationship between glioma and sarcopenia. The study identified eight co-downregulated miRNAs, three co-upregulated miRNAs, and seven genes associated with overall glioma survival, namely, KRAS, IFNB1, ALCAM, ERBB2, STAT3, FOSL1, and EN2. With a multi-factor Cox regression model incorporating FOSL1 and EN2, we obtained ROC curves of 0.702 and 0.709, respectively, suggesting that glioma prognosis can be predicted by FOSL1 and EN2, which are differentially expressed in both cancer and aged muscle. FOSL1 and EN2 were analyzed using Gene Set Enrichment Analysis to identify possible functional pathways. RT-qPCR and a dual-luciferase reporter gene system verified that hsa-miR-33a targets FOSL1 and EN2. We found that hsa-mir-33a co-targeting FOSL1 and EN2 has a good predictive value for glioblastoma and skeletal muscle reduction.
为了确定胶质瘤与肌肉衰老之间的关系，并通过筛选共表达基因来预测预后，本研究检测了胶质瘤与肌肉减少症之间的关系。该研究鉴定出8个共下调的miRNA、3个共上调的miRNA以及7个与胶质瘤总体生存相关的基因，即KRAS、IFNB1、ALCAM、ERBB2、STAT3、FOSL1和EN2。通过纳入FOSL1和EN2的多因素Cox回归模型，我们分别获得了0.702和0.709的ROC曲线，表明FOSL1和EN2可预测胶质瘤预后，这两个基因在癌症和衰老肌肉中均有差异表达。使用基因集富集分析对FOSL1和EN2进行分析，以确定可能的功能途径。RT-qPCR和双荧光素酶报告基因系统验证了hsa-miR-33a靶向FOSL1和EN2。我们发现，共同靶向FOSL1和EN2的hsa-mir-33a对胶质母细胞瘤和骨骼肌减少具有良好的预测价值。
