Abdel Fadeel Doaa A, Hanafy Magda S, Kelany Nermeen A, Elywa Mohammed A
Pharmaceutical Technology Unit, Department of Medical Applications of Laser, National Institute of Laser Enhanced Sciences, Cairo University, Cairo, Egypt.
Biophysics Branch, Physics Department, Faculty of Science, Zagazig University, 44519 Zagazig, Egypt.
Heliyon. 2021 Jun 21;7(6):e07370. doi: 10.1016/j.heliyon.2021.e07370. eCollection 2021 Jun.
In a previous work, a pure crystalline titanium dioxide nanoparticles (TiONPs) were synthesized by green synthesis technique using Aloe vera leaves extract as reducing agent. In this work, we are aiming to investigate the potential of the novel greenly synthesized TiONPs as a nano-drug delivery system for the anticancer drug, doxorubicin (Dox).
The cytotoxicity of the synthesized TiONPs was tested on two cell lines; normal human skin fibroblasts (HSF) and breast adenocarcinoma cells (MCF-7). Then, Dox was loaded to both TiONPs (Dox- TiONPs) and liposomes (Dox-Lip). The loaded nanoparticles were characterized by TEM, FTIR, encapsulation efficiency, particle size and zeta potential measurement. Moreover, in vitro drug release was studied. Ehrlich tumor-bearing mice were used to study the anticancer activity of Dox- TiONPs, Dox-Lip, and aqueous Dox solution. Tumor volume, survival rate, and histopathological alterations were compared in all groups.
Dox was successfully loaded to both liposomes and TiONPs with an encapsulation efficiency of 77% and 65%, respectively. The particle size of Dox-TiONPs, and Dox-Lip was 14.53 nm, and 103 nm, respectively. The cumulative Dox released from TiONPs and liposomes after 4 h was 18 and 46%, respectively.Dox-Lip and Dox-TiONPs resulted in the highest degree of tumor growth inhibition with 100% and 83% of treated animals remained alive, respectively.
The greenly synthesized TiONPs were proved to be as effective as liposomes in enhancing the anticancer activity of Dox.
在之前的一项工作中，采用绿色合成技术，以芦荟叶提取物作为还原剂，合成了纯结晶二氧化钛纳米颗粒（TiONPs）。在本研究中，我们旨在探究新型绿色合成的TiONPs作为抗癌药物阿霉素（Dox）纳米药物递送系统的潜力。
在两种细胞系上测试合成的TiONPs的细胞毒性；正常人皮肤成纤维细胞（HSF）和乳腺腺癌细胞（MCF-7）。然后，将阿霉素分别负载到TiONPs（阿霉素-TiONPs）和脂质体（阿霉素-脂质体）中。通过透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、包封率、粒径和zeta电位测量对负载的纳米颗粒进行表征。此外，还研究了体外药物释放。使用荷艾氏腹水癌小鼠研究阿霉素-TiONPs、阿霉素-脂质体和阿霉素水溶液的抗癌活性。比较所有组的肿瘤体积、存活率和组织病理学改变。
阿霉素成功负载到脂质体和TiONPs中，包封率分别为77%和65%。阿霉素-TiONPs和阿霉素-脂质体的粒径分别为14.53nm和103nm。4小时后从TiONPs和脂质体中释放的阿霉素累积量分别为18%和46%。阿霉素-脂质体和阿霉素-TiONPs导致最高程度的肿瘤生长抑制，分别有100%和83%的治疗动物存活。
绿色合成的TiONPs在增强阿霉素的抗癌活性方面被证明与脂质体一样有效。
