Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA.
Methods Mol Biol. 2021;2314:167-182. doi: 10.1007/978-1-0716-1460-0_6.
Mycobacterium tuberculosis colonizes, survives, and grows inside macrophages. In vitro macrophage infection models, using both primary macrophages and cell lines, enable the characterization of the pathogen response to macrophage immune pressure and intracellular environmental cues. We describe methods to propagate and infect primary murine bone marrow-derived macrophages, HoxB8 conditionally immortalized myeloid cells, Max Planck Institute alveolar macrophage-like cells, and J774 and THP-1 macrophage-like cell lines. We also present methods on the characterization of M. tuberculosis intracellular survival and the preparation of infected macrophages for imaging.
结核分枝杆菌在巨噬细胞内定殖、存活和生长。使用原代巨噬细胞和细胞系的体外巨噬细胞感染模型，使我们能够描述病原体对巨噬细胞免疫压力和细胞内环境信号的反应。我们描述了增殖和感染原代鼠骨髓来源的巨噬细胞、HoxB8 条件永生化髓样细胞、马克斯·普朗克肺巨噬细胞样细胞、J774 和 THP-1 巨噬细胞样细胞系的方法。我们还介绍了用于表征结核分枝杆菌细胞内存活和准备感染巨噬细胞进行成像的方法。
