Key Laboratory of Molecular Target & Clinical Pharmacology and the State & NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, PR China.
Key Laboratory of Molecular Target & Clinical Pharmacology and the State & NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, PR China.
J Colloid Interface Sci. 2021 Dec;603:822-833. doi: 10.1016/j.jcis.2021.06.154. Epub 2021 Jun 30.
Herein, N-doped porous carbon nano-polyhedron embedded with CoO (CoO-NPCN) was reported for the electro-catalytic oxidation and amperometric detection of l-cysteine. CoO-NPCN was synthesized by the two-step redox calcination of zeolitic imidazolate framework (ZIF). Surface morphology characterization revealed that CoO-NPCN displayed a uniform size and rhombic dodecahedral shape. Structure and composition analysis found that CoO-NPCN was a N-doped carbon polyhedral matrix with hollow and porous structure, and CoO nano-spheres were evenly distributed into the polyhedral matrix. Due to the hollow and porous structure, N-doped carbon matrix and embedded CoO nano-spheres, CoO-NPCN performed a remarkable electro-catalysis towards the oxidation of l-cysteine at a very low potential of 0.10 V. A diffusion-controlled l-cysteine oxidation process was observed at CoO-NPCN prepared electrode. Accordingly, amperometric method was established for l-cysteine detection with a very fast current response in 2 s, wide linear range of 0.05 μM- 5.2 mM and low detection limit of 6.9 nM. Besides, notable selectivity, repeatability, reproducibility and long-term stability were also achieved. Moreover, CoO-NPCN sensor was successfully applied to the l-cysteine detection in human serum samples indicating the practical application of the as-developed sensor.
本文报道了一种氮掺杂多孔碳纳米多面体嵌入 CoO（CoO-NPCN），用于 l-半胱氨酸的电催化氧化和安培检测。CoO-NPCN 通过沸石咪唑酯骨架（ZIF）的两步氧化还原煅烧合成。表面形貌表征显示 CoO-NPCN 呈现均匀的尺寸和菱形十二面体形状。结构和组成分析发现，CoO-NPCN 是一种具有中空和多孔结构的 N 掺杂碳多面体基体，CoO 纳米球均匀分布在多面体基体中。由于具有中空和多孔结构、N 掺杂碳基体和嵌入的 CoO 纳米球，CoO-NPCN 对 l-半胱氨酸的氧化表现出非常低的 0.10 V 电位下的显著电催化作用。在 CoO-NPCN 制备的电极上观察到 l-半胱氨酸的扩散控制氧化过程。因此，建立了安培法用于 l-半胱氨酸检测，具有非常快的电流响应（2 s）、宽线性范围（0.05 μM-5.2 mM）和低检测限（6.9 nM）。此外，还实现了显著的选择性、重复性、重现性和长期稳定性。此外，CoO-NPCN 传感器成功应用于人血清样品中的 l-半胱氨酸检测，表明所开发传感器的实际应用。
