Department of Biotechnology, GMU-GIBH Joint School of Life Science, Guangzhou Medical University, Guangzhou, 511436, PR China.
Key Laboratory of Molecular Target & Clinical Pharmacology and the State & NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, 511436, PR China.
Anal Chim Acta. 2022 May 29;1209:339839. doi: 10.1016/j.aca.2022.339839. Epub 2022 Apr 16.
The global prevalence of diabetes makes it a significant work to develop flagship sensors in glucose monitoring technology. Particularly, exploring highly active nanocomposites as biomimetic catalysts for the enzymatic reaction of glucose is extremely attractive in non-enzymatic glucose sensing. Herein, nitrogen-doped hollow carbon nano-polyhedron implanted with CoO nanoparticles (NHCN-CoO) was introduced as nanozyme for the catalytic oxidation of glucose. NHCN-CoO was synthesized by a two-step redox carbonization of zeolitic imidazolate frameworks. Morphology and structure characterizations revealed that NHCN-CoO was a rhombic nano-dodecahedron with hollow N-doped carbon frameworks. In the frameworks, well-defined CoO nanoparticles were embedded. With highly porous N-doped graphitization structure and embedded CoO, NHCN-CoO displayed a distinguished biomimetic catalysis towards the direct oxidation of glucose at a low onset potential of 0.30 V. The biomimetic catalysis of glucose oxidation at NHCN-CoO was so efficient that a steady-state current signal could be established within 3 s. By using NHCN-CoO as nanozyme, a brilliant non-enzymatic glucose sensor was developed with a very low detection limit of 0.2 μM and broad detection range from 1.0 μM to 32.0 mM. Besides, NHCN-CoO sensor also displayed an effective anti-interference capability towards the simulated interfering species including small biomolecules, amino acids, and chloride ion. Furthermore, notable repeatability, reproducibility and long-term stability were also presented. Finally, the successful blood sugar detection in human serum strongly manifests the possible real application of NHCN-CoO sensor.
全球糖尿病的患病率使得开发葡萄糖监测技术中的旗舰传感器成为一项重要工作。特别是，探索作为葡萄糖酶促反应的仿生催化剂的高活性纳米复合材料在非酶葡萄糖传感中极具吸引力。在此，引入氮掺杂空心碳纳米多面体嵌入 CoO 纳米粒子（NHCN-CoO）作为纳米酶用于葡萄糖的催化氧化。NHCN-CoO 通过沸石咪唑酯骨架的两步还原碳化合成。形貌和结构表征表明，NHCN-CoO 是具有空心 N 掺杂碳骨架的菱形十二面体纳米晶。在骨架中，嵌入了定义明确的 CoO 纳米粒子。NHCN-CoO 具有高度多孔的 N 掺杂石墨化结构和嵌入的 CoO，对葡萄糖的直接氧化表现出出色的仿生催化作用，其起始电位低至 0.30 V。在 NHCN-CoO 上葡萄糖氧化的仿生催化作用非常高效，在 3 s 内即可建立稳定的电流信号。通过将 NHCN-CoO 用作纳米酶，开发了一种出色的非酶葡萄糖传感器，其检测限非常低，为 0.2 μM，检测范围从 1.0 μM 到 32.0 mM 很宽。此外，NHCN-CoO 传感器对包括小分子生物、氨基酸和氯离子在内的模拟干扰物质也表现出有效的抗干扰能力。此外，还呈现出良好的重复性、重现性和长期稳定性。最后，在人血清中成功进行血糖检测强烈表明 NHCN-CoO 传感器具有潜在的实际应用。
