Peavy D L, Baughn R E, Musher D M
Infect Immun. 1978 Jan;19(1):71-8. doi: 10.1128/iai.19.1.71-78.1978.
The in vivo effect of bacterial lipopolysaccharides (LPS) on mouse spleen cell subpopulations was investigated. Intravenous administration of LPS resulted in marked enlargement of the spleen, accompanied by increased cellular proliferation and enhanced nucleated cell recoveries. At least two morphologically distinct cell types appeared to be targets for LPS. Polymorphonuclear leukocytes accumulated rapidly with a relatively minor degree of cell division. In contrast, a substantial proportion of splenic lymphocytes transformed into large lymphocytes and blast cells which actively incorporated [3H]thymidine. Proliferating cells were identified as bone marrow-derived (B) lymphocytes by their ability to form C3-dependent rosettes and to synthesize immunoglobulin. These cellular responses were not antigenically induced, since LPS derived from mutants lacking the polysaccharide moiety gave similar results. Thus, splenic B lymphocytes appear to interact and respond to LPS in vivo in the same manner as observed in vitro. These data suggest that the capacity of LPS to directly activate B lymphocytes, initiate cellular proliferation, and induce immunoglobulin production by bone marrow-derived cells in vivo may contribute to its adjuvant activity.
研究了细菌脂多糖（LPS）对小鼠脾细胞亚群的体内作用。静脉注射LPS导致脾脏明显肿大，伴有细胞增殖增加和有核细胞回收率提高。至少两种形态上不同的细胞类型似乎是LPS的作用靶点。多形核白细胞迅速聚集，细胞分裂程度相对较小。相比之下，相当一部分脾淋巴细胞转化为大淋巴细胞和母细胞，这些细胞能积极掺入[3H]胸腺嘧啶核苷。通过形成C3依赖性玫瑰花结和合成免疫球蛋白的能力，增殖细胞被鉴定为骨髓来源的（B）淋巴细胞。这些细胞反应不是由抗原诱导的，因为来自缺乏多糖部分的突变体的LPS也给出了类似的结果。因此，脾B淋巴细胞在体内似乎以与体外观察到的相同方式与LPS相互作用并对其作出反应。这些数据表明，LPS在体内直接激活B淋巴细胞、启动细胞增殖以及诱导骨髓来源细胞产生免疫球蛋白的能力可能有助于其佐剂活性。
