Wang Yingjun, Wu Mengting, Tang Panyang, Jiang Dongmei
College of Environment, Sichuan Agricultural University, Chengdu 611130, China.
Gels. 2024 Sep 13;10(9):591. doi: 10.3390/gels10090591.
In this study, we used chitosan/sodium alginate hydrogel as a carrier to prepare berberine sustained-release capsule materials that can inhibit algae for a long time and safely. The preparation conditions of the material were optimized by the response surface method, and the optimized capsule material was characterized and the sustained release characteristics were analyzed to study the change of the algae inhibition effect of the material within 30 days. The results showed that the optimum preparation parameters of the material were 0.54% chitosan content, 2.46% sodium alginate content and 1.09% anhydrous calcium chloride content by response surface optimization design, which was consistent with the parameters set by each factor at the central point. The algae inhibition rate of the material under this preparation condition was 93.75 ± 1.01%, which was similar to the predicted value. The release characteristics analysis showed that the material continuously released up to 90% of berberine within 24 days, and its release characteristics were sustained release after burst release, with good sustained release effect. The results of material characterization showed that chitosan/sodium alginate hydrogel could effectively load berberine and was beneficial to the loading and release of berberine. The results of algae inhibition experiments showed that low concentration materials could control the outbreak of cyanobacterial blooms in a short time, while under high concentration conditions, the materials could inhibit efficiently and for a long time.
在本研究中，我们使用壳聚糖/海藻酸钠水凝胶作为载体来制备能够长期且安全地抑制藻类的黄连素缓释胶囊材料。通过响应面法对材料的制备条件进行优化，对优化后的胶囊材料进行表征并分析其缓释特性，以研究该材料在30天内藻类抑制效果的变化。结果表明，通过响应面优化设计得到的材料最佳制备参数为壳聚糖含量0.54%、海藻酸钠含量2.46%、无水氯化钙含量1.09%，这与各因素在中心点设定的参数一致。在此制备条件下材料的抑藻率为93.75±1.01%，与预测值相近。释放特性分析表明，该材料在24天内持续释放黄连素高达90%，其释放特性为突释后缓释，缓释效果良好。材料表征结果表明，壳聚糖/海藻酸钠水凝胶能够有效地负载黄连素，有利于黄连素的负载和释放。藻类抑制实验结果表明，低浓度材料可在短时间内控制蓝藻水华的爆发，而在高浓度条件下，该材料能够高效且长期地抑制藻类。
