Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia.
Division of Endocrinology, Department of Internal Medicine, Dr. Cipto Mangunkusumo National Referral Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia.
F1000Res. 2021 Feb 8;10:79. doi: 10.12688/f1000research.28216.4. eCollection 2021.
: Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and determining host response. As such, a study of changing subgingival biofilms is important for understanding the effect of a systemic condition. In this study, we compared the occurrence of six bacteria cohabiting in the subgingival area of periodontitis subjects, with (DP, n = 8) and without (NDP, n = 4) diabetes. : The six genus and species of targeted bacteria were confirmed by 16S rRNA amplicon sequencing on MinION nanopore platform. Descriptive statistic was used to describe the obtained data. : We found that the six genus and species of targeted bacteria were detected but in different quantities in either group's periodontal pocket. Our data showed that Tannerella forsythia was the most abundant species in subgingival biofilms of the DP group of the red complex bacteria. In contrast, Aggregatibacter sp., which belongs to the phylum of proteobacteria, was present at a relatively lower level. In contrast, Fusobacterium sp., which belongs to orange complex bacteria, showed relative similarities in subgingival biofilms of both groups tested, while Veillonella sp., were abundant in the DP groups. Our data show that the diversity of classic periodontopathogens increased in the subgingival niche of periodontitis subjects with diabetes. It is the first study in Indonesia to apply MinION-based, full-length 16S rRNA amplicon sequencing in periodontitis patients with and without diabetes.
龈下生态位是富含微生物群的生物膜栖息地之一，它在维持牙周组织健康和决定宿主反应方面发挥着积极作用。因此，研究龈下生物膜的变化对于理解全身状况的影响很重要。在这项研究中，我们比较了患有和不患有糖尿病（DP，n=8 和 NDP，n=4）的牙周炎患者龈下区域共栖的六种细菌的发生情况。六种目标细菌的属和种通过 MinION 纳米孔平台上的 16S rRNA 扩增子测序来确认。采用描述性统计来描述获得的数据。我们发现，这六种目标细菌的属和种都被检测到，但在两组牙周袋中的数量不同。我们的数据显示，Tannerella forsythia 是 DP 组红色复合体细菌龈下生物膜中最丰富的物种。相比之下，属于变形菌门的 Aggregatibacter sp. 的含量相对较低。相反，属于橙色复合体细菌的 Fusobacterium sp. 在两组测试的龈下生物膜中表现出相对相似性，而 Veillonella sp. 在 DP 组中含量丰富。我们的数据表明，糖尿病牙周炎患者龈下生态位中经典牙周病原体的多样性增加。这是印度尼西亚首次在患有和不患有糖尿病的牙周炎患者中应用基于 MinION 的全长 16S rRNA 扩增子测序。
