Division of Periodontics, Paulista University, São Paulo, Brazil.
J Periodontal Res. 2013 Feb;48(1):30-6. doi: 10.1111/j.1600-0765.2012.01498.x. Epub 2012 Jul 4.
There is a bidirectional relationship between periodontal disease and type-2 diabetes mellitus (DM). Inflammatory mediators may negatively affect glycemic control, and increased glucose levels and resultant glycation end-products may alter the host response against bacterial infection. However, no agreement has been reached regarding the effect of DM on periodontal subgingival microbiota. Therefore, the purpose of the present study was to compare the subgingival biodiversity in deep periodontal pockets of subjects with chronic periodontitis and either uncontrolled type-2 diabetes or no diabetes using 16S rRNA gene cloning and sequencing.
Twelve subjects with uncontrolled type-2 diabetes (glycated hemoglobin > 8%) and eleven nondiabetic subjects presenting severe and generalized chronic periodontitis were selected. Subgingival biofilm from periodontal pockets > 5 mm were assessed using the 16S rRNA gene cloning and sequencing technique.
Significant differences were observed in subgingival microbiota between diabetic and nondiabetic subjects. Diabetic subjects presented higher percentages of total clones of TM7, Aggregatibacter, Neisseria, Gemella, Eikenella, Selenomonas, Actinomyces, Capnocytophaga, Fusobacterium, Veillonella and Streptococcus genera, and lower percentages of Porphyromonas, Filifactor, Eubacterium, Synergistetes, Tannerella and Treponema genera than nondiabetic individuals (p < 0.05). Moreover, some phylotypes, such as Fusobacterium nucleatum, Veillonella parvula, V. dispar and Eikenella corrodens were detected significantly more often in diabetic subjects than in nondiabetic subjects (p < 0.05).
Subjects with uncontrolled type-2 diabetes and chronic periodontitis presented significant dissimilarities in subgingival biodiversity compared with nondiabetic subjects.
牙周病和 2 型糖尿病（DM）之间存在双向关系。炎症介质可能会对血糖控制产生负面影响，而升高的葡萄糖水平和由此产生的糖基化终产物可能会改变宿主对细菌感染的反应。然而，对于 DM 对牙周龈下微生物群的影响，尚未达成共识。因此，本研究旨在使用 16S rRNA 基因克隆和测序比较患有慢性牙周炎且血糖控制不佳的 2 型糖尿病患者和无糖尿病患者的深牙周袋龈下生物多样性。
选择 12 名血糖控制不佳（糖化血红蛋白>8%）的 2 型糖尿病患者和 11 名患有严重和广泛慢性牙周炎的非糖尿病患者。使用 16S rRNA 基因克隆和测序技术评估牙周袋>5mm 的龈下生物膜。
糖尿病患者和非糖尿病患者的龈下微生物群存在显著差异。糖尿病患者的 TM7、Aggregatibacter、Neisseria、Gemella、Eikenella、Selenomonas、Actinomyces、Capnocytophaga、Fusobacterium、Veillonella 和 Streptococcus 属的总克隆百分比较高，而 Porphyromonas、Filifactor、Eubacterium、Synergistetes、Tannerella 和 Treponema 属的总克隆百分比较低（p<0.05）。此外，一些菌株，如 Fusobacterium nucleatum、Veillonella parvula、V. dispar 和 Eikenella corrodens，在糖尿病患者中的检出率明显高于非糖尿病患者（p<0.05）。
与非糖尿病患者相比，血糖控制不佳的 2 型糖尿病合并慢性牙周炎患者的龈下生物多样性存在显著差异。
