Matsukawa Hiroki, Iida Noriho, Kitamura Kazuya, Terashima Takeshi, Seishima Jun, Makino Isamu, Kannon Takayuki, Hosomichi Kazuyoshi, Yamashita Taro, Sakai Yoshio, Honda Masao, Yamashita Tatsuya, Mizukoshi Eishiro, Kaneko Shuichi
Department of Gastroenterology, Graduate School of Medical Sciences, Kanazawa University 13-1 Takara-Machi, Kanazawa, Ishikawa, Japan.
Department of Hepato-Biliary-Pancreatic Surgery and Transplantation, Graduate School of Medical Sciences, Kanazawa University 13-1 Takara-Machi, Kanazawa, Ishikawa, Japan.
Am J Cancer Res. 2021 Jun 15;11(6):3163-3175. eCollection 2021.
Microbiota in the gut and oral cavities of pancreatic cancer (PC) patients differ from those of healthy persons, and bacteria in PC tissues are associated with patients' prognoses. However, the species-level relationship between a dysbiotic gut, oral and cancerous microbiota, and prognostic factors remains unknown. Whole-genome sequencing was performed with fecal DNA from 24 PC patients and 18 healthy persons (HD). Microbial taxonomies, metabolic pathways, and viral presence were determined. DNA was sequenced from saliva and PC tissues, and the association between the gut, oral, and cancer microbiota and prognostic factors in PC patients was analyzed. The PC microbiota were altered from those of the healthy microbiota in terms of microbial taxonomy, pathways and viral presence. Twenty-six species differed significantly between the PC and HD microbiota. Six fecal microbes, including , were associated with an increased hazard of death. In the co-occurrence network, microbes that were abundant in PC patients were plotted close together and formed clusters with prognosis-associated microbes, including . Multiple salivary microbes were present in the co-occurrence network. and were detected in the PC tissues and formed a network with the fecal and salivary microbes. The dysbiotic gut microbiota in the PC patients formed a complex network with the oral and cancerous microbiota, and gut microbes abundant in the PC patients were closely linked with poor prognostic factors in the network.
胰腺癌（PC）患者肠道和口腔中的微生物群与健康人不同，PC组织中的细菌与患者预后相关。然而，失调的肠道、口腔和癌性微生物群之间的物种水平关系以及预后因素仍不清楚。对24例PC患者和18例健康人（HD）的粪便DNA进行了全基因组测序。确定了微生物分类学、代谢途径和病毒存在情况。对唾液和PC组织的DNA进行了测序，并分析了PC患者肠道、口腔和癌性微生物群与预后因素之间的关联。PC微生物群在微生物分类学、途径和病毒存在方面与健康微生物群有所不同。PC和HD微生物群之间有26种显著差异。六种粪便微生物，包括 ，与死亡风险增加相关。在共现网络中，PC患者中丰富的微生物聚集在一起，并与包括 在内的预后相关微生物形成簇。共现网络中存在多种唾液微生物。 在PC组织中被检测到，并与粪便和唾液微生物形成一个网络。PC患者中失调的肠道微生物群与口腔和癌性微生物群形成了一个复杂的网络，PC患者中丰富的肠道微生物在网络中与不良预后因素密切相关。
