prevalence in healthy Mexican children: comparison between two non-invasive methods.

BACKGROUND

detection in asymptomatic children with suspected infection or with symptoms that suggest gastric pathology is problematic, since most of the methods depend on the endoscopic study, an invasive and expensive method. Non-invasive methods can be a feasible alternative but must be validated. The purpose of this study was to evaluate the concordance between DNA detection in saliva and dental plaque by PCR, with antigen detection in stool by immunochromatography, among asymptomatic children in the state of Guerrero, Mexico.

METHODS

Dental plaque, saliva, and stool samples were obtained from 171 children between 6 and 12 years old. detection in saliva and dental plaque was performed by PCR using specific primers for the 16S rRNA gene, while the detection in stool samples was performed by immunochromatography using the CerTest kit.

RESULTS

We found an overall prevalence of 59.6% (102/171). Of the positive children 18% (20/111) were positive in saliva samples, 28.1% (34/121) in dental plaque samples, and 50.4% (71/141) in stool samples. A higher prevalence was found in girls (64.7%, = 0.002). Although some of the children declared some dyspeptic symptoms, these were no related to . In conclusion, we found a high prevalence of in asymptomatic children and the highest proportion was detected by stool antigen test, which was the most feasible method to detect infection.