Park Beom Kyun, Son Dong Chan, Ghimire Balkrishna
Division of Forest Biodiversity, Korea National Arboretum, Pocheon, South Korea.
PeerJ. 2021 Jun 29;9:e11669. doi: 10.7717/peerj.11669. eCollection 2021.
We assessed the micro-morphological and anatomical structures of the petioles of 19 taxa from South Korea. The petiole surface features were observed with the help of stereomicroscopy and scanning electron microscopy, and the anatomical features are studied via microtomy and light microscopy. The results of this study showed that the presence/absence and abundance of trichomes, petiole cross-section outlines, upper surface wings and grooves, and the number of vascular bundles were useful for species discrimination in . Among the studied taxa, was the only species with a glabrous petiole surface. Two types of trichomes were observed in the other 18 taxa: long, non-glandular and flagelliform trichomes and short, glandular capitate trichomes. We found four to six major vascular bundles and a maximum of eight interfascicular vascular bundles ( and ) in the 19 taxa. A cluster analysis based on UPGMA identified six clusters with 18 nodes. Although the number of taxa investigated was limited, taxa from the sections , , and clustered with each other in the UPGMA phenogram due to the overall similarity of petiole features. Based on this observation, we can conclude that most of the petiole features are limited to the species level, and thus, the data obtained could be used as descriptive and/or diagnostic features for particular taxa, which may be useful for the investigation of problematic taxa in the genus.
我们评估了来自韩国的19个分类群叶柄的微观形态和解剖结构。借助体视显微镜和扫描电子显微镜观察叶柄表面特征，并通过切片术和光学显微镜研究解剖特征。本研究结果表明，毛状体的有无和丰度、叶柄横截面轮廓、上表面翅和凹槽以及维管束数量有助于在……中进行物种鉴别。在所研究的分类群中，……是唯一叶柄表面无毛的物种。在其他18个分类群中观察到两种类型的毛状体：长的、非腺毛状鞭状毛状体和短的、腺头状毛状体。我们在19个分类群中发现了四到六个主要维管束，最多八个束间维管束（……和……）。基于UPGMA的聚类分析确定了六个聚类，有18个节点。尽管所研究的分类群数量有限，但由于叶柄特征的总体相似性，来自……、……和……组的分类群在UPGMA系统发育图中彼此聚类。基于这一观察结果，我们可以得出结论，大多数叶柄特征仅限于物种水平，因此，所获得的数据可以用作特定分类群的描述性和/或诊断性特征，这可能有助于研究该属中有问题的分类群。
