Ishtiaq C Muhammad, He Q, Feng S, Wang Yi, Xiao P G, Cheng Yi Yu
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Institute of Bioinformatics, Zhejiang University, No. 38, Zhe da Road Hangzhou, 310027, People's Republic of China.
Pak J Biol Sci. 2007 Jun 15;10(12):2066-72. doi: 10.3923/pjbs.2007.2066.2072.
Distribution patterns of chemical compounds in plants have been used for biosystematic and phylogenetic studies. Saponin profile of twelve major taxa of Clematis genus, belonging to sections, Rectae, Clematis, Meclatis, Tubulosae and Viorna were analyzed by HPLC coupled with diode array detector and ESI-MS. The chemodiversity profile of saponins has unambiguously delimited the taxa of Clematis at subgenus, section and subsection level. The distribution of saponins in Clematis genus provides useful taxonomic markers and results are presented in phenograms. The compound Huzhangoside D was common and the most abundant in analyzed species of the genus. The morphological analysis was also conducted of the same taxa and presented as cluster tree. The distribution and chemotaxonomic importance of saponins profile within the genus is discussed.
植物中化合物的分布模式已被用于生物系统学和系统发育研究。采用高效液相色谱联用二极管阵列检测器和电喷雾电离质谱法，对铁线莲属十二个主要类群（属于直花铁线莲组、铁线莲组、准葛尔铁线莲组、尾叶铁线莲组和长瓣铁线莲组）的皂苷谱进行了分析。皂苷的化学多样性谱已明确界定了铁线莲属在亚属、组和亚组水平上的类群。铁线莲属中皂苷的分布提供了有用的分类标记，并以聚类图的形式呈现了结果。化合物虎杖苷D在所分析的该属物种中普遍存在且含量最高。还对相同类群进行了形态学分析，并呈现为聚类树。讨论了该属内皂苷谱的分布及其化学分类学重要性。
