Ishiwata I, Ishiwata C, Soma M, Nozawa S, Ishikawa H
Ishiwata Obstetrical and Gynecological Hospital, Mito, Japan.
Acta Cytol. 1987 Nov-Dec;31(6):925-34.
The cell of origin of uterine cervical cancer was studied by using culture, enzyme histochemistry and heterotransplantation. Twenty-seven epidermoid carcinomas (8 large cell keratinizing squamous, 12 large cell nonkeratinizing squamous and 7 small cell nonkeratinizing squamous) and 2 adenocarcinomas of the uterine cervix were placed in culture. An outgrowth of carcinoma cells in vitro was observed in 22 of 29 cases: 6 keratinizing, 8 large cell nonkeratinizing and 6 small cell nonkeratinizing carcinomas and 2 adenocarcinomas. The squamous carcinomas showed a squamous-cell outgrowth pattern, except for one large cell nonkeratinizing and three small cell nonkeratinizing carcinomas that showed a glandular-cell outgrowth pattern. One of three keratinizing carcinomas was transplantable into the subcutis of BALB/c nude mice, producing keratinizing tumors; three of six large cell and one of three small cell nonkeratinizing carcinomas reproduced themselves, while the other two small cell carcinomas produced poorly differentiated adenocarcinomas in mice. The transplanted adenocarcinoma produced a well-differentiated adenocarcinoma resembling the original tumor. Small cell carcinomas and adenocarcinomas contained a heat-stable, L-phenylalanine-sensitive alkaline phosphatase. These results suggest that many uterine cervical cancers originate from the reserve cell.
采用培养、酶组织化学和异种移植方法对子宫颈癌的起源细胞进行了研究。将27例表皮样癌（8例大细胞角化性鳞状细胞癌、12例大细胞非角化性鳞状细胞癌和7例小细胞非角化性鳞状细胞癌）和2例子宫颈腺癌进行培养。29例中有22例观察到癌细胞在体外生长：6例角化性癌、8例大细胞非角化性癌、6例小细胞非角化性癌和2例腺癌。鳞状细胞癌表现为鳞状细胞生长模式，但有1例大细胞非角化性癌和3例小细胞非角化性癌表现为腺细胞生长模式。3例角化性癌中有1例可移植到BALB/c裸鼠皮下，产生角化性肿瘤；6例大细胞非角化性癌中的3例和3例小细胞非角化性癌中的1例在小鼠体内自行复制，而另外2例小细胞癌在小鼠体内产生低分化腺癌。移植的腺癌产生了与原发肿瘤相似的高分化腺癌。小细胞癌和腺癌含有一种热稳定的、对L-苯丙氨酸敏感的碱性磷酸酶。这些结果提示许多子宫颈癌起源于储备细胞。
