Iwasaka T, Kidera Y, Tsugitomi H, Sugimori H
Department of Obstetrics and Gynecology, Saga Medical School, Japan.
Acta Cytol. 1987 Nov-Dec;31(6):935-40.
The cellular changes of primary and recurrent herpes simplex virus (HSV) infection were investigated in in vitro models. In the primary infection model, nuclear changes were characterized by clumping and margination of the nuclear chromatin, a homogeneous ground-glass nuclear appearance, multinucleation and the appearance of two different types of intranuclear inclusions. One of the two types of inclusions appeared as early as six hours postinfection, reached a maximum at nine hours postinfection and gradually decreased thereafter. This early inclusion, relatively small in size as compared to the other type of inclusion, gave a basophilic staining when the Papanicolaou staining method was used. The other type of inclusion was the typical Cowdry type A inclusion, which appeared as early as 12 hours postinfection. Both types of inclusions contained clear perinuclear halo. In the recurrent infection model, the appearance of all the nuclear changes was delayed, the appearance of early inclusions was infrequent, and the Cowdry type A inclusions were observed more frequently than in the primary infection model. These results may indicate that the early inclusion is a sign of rapid virus replication while the Cowdry type A inclusion is one form of the remains of an HSV infection.
在体外模型中研究了原发性和复发性单纯疱疹病毒（HSV）感染的细胞变化。在原发性感染模型中，细胞核变化的特征为核染色质的凝聚和边缘化、均匀的毛玻璃样核外观、多核化以及出现两种不同类型的核内包涵体。两种类型的包涵体之一在感染后6小时最早出现，在感染后9小时达到最大值，此后逐渐减少。与另一种类型的包涵体相比，这种早期包涵体尺寸相对较小，采用巴氏染色法时呈嗜碱性染色。另一种类型的包涵体是典型的考德里A型包涵体，最早在感染后12小时出现。两种类型的包涵体均含有清晰的核周晕。在复发性感染模型中，所有细胞核变化的出现均延迟，早期包涵体的出现不常见，且考德里A型包涵体的观察频率高于原发性感染模型。这些结果可能表明，早期包涵体是病毒快速复制的标志，而考德里A型包涵体是HSV感染残留物的一种形式。
