Girardi E, Rodríguez de Lores Arnaiz G
Instituto de Biología Celular, Facultad de Medicina, Buenos Aires, Argentina.
Acta Physiol Pharmacol Latinoam. 1987;37(2):235-43.
Systemic administration of bicuculline (Bic) and 3-mercaptopropionic acid (MP) induces generalized seizure in laboratory animals. The effect of these convulsants on lactate dehydrogenase and citrate synthase activities in rat cerebral cortex was studied. Bic was administered i.p. at 1.0 mg/kg (subconvulsant dose) and 7.5 mg/kg (convulsant dose). MP was administered i.p. at 150 mg/kg (convulsant dose). Lactate dehydrogenase activity in cerebral cortex soluble and particulated fractions was not modified by the administration of Bic or MP. Citrate synthase activity in cerebral cortex homogenates increased about 40% by the administration of subconvulsant and convulsant doses of Bic. A similar increase was found after the injection of MP. No changes in the liver enzyme activity were found, suggesting tissue specificity. The finding of a higher activity of citrate synthase in cerebral cortex homogenates observed with the convulsants may be correlated with the increase in brain citrate levels reported in seizure states.
对实验动物进行荷包牡丹碱（Bic）和3-巯基丙酸（MP）的全身给药会诱发全身性癫痫发作。研究了这些惊厥剂对大鼠大脑皮层中乳酸脱氢酶和柠檬酸合酶活性的影响。荷包牡丹碱以1.0毫克/千克（亚惊厥剂量）和7.5毫克/千克（惊厥剂量）腹腔注射给药。3-巯基丙酸以150毫克/千克（惊厥剂量）腹腔注射给药。荷包牡丹碱或3-巯基丙酸的给药并未改变大脑皮层可溶性和颗粒部分中的乳酸脱氢酶活性。亚惊厥剂量和惊厥剂量的荷包牡丹碱给药使大脑皮层匀浆中的柠檬酸合酶活性增加了约40%。注射3-巯基丙酸后也发现了类似的增加。未发现肝脏酶活性有变化，提示存在组织特异性。惊厥剂作用下观察到大脑皮层匀浆中柠檬酸合酶活性较高这一发现可能与癫痫发作状态下大脑柠檬酸水平的升高有关。
