Rao Jun, Fu Jinjin, Meng Chuchen, Huang Jin, Qin Xiangrong, Zhuang Shaohua
Department of Gastroenterology, Changzhou No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, No. 29 Xinglong District, Changzhou 213000, China.
Department of Endocrinology, Changzhou No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, Changzhou, China.
J Oncol. 2021 Jun 28;2021:9935410. doi: 10.1155/2021/9935410. eCollection 2021.
The function and possible mechanism of lncRNA Small Nucleolar RNA Host Gene 3 (SNHG3) in GC have not been fully studied. The aim of our study was to investigate the role of SNHG3 in the proliferation, migration, and invasion of GC cell lines. The expressions of SNHG3, miR-326, and TWIST in GC9811-P GC cell lines were detected by RT-qPCR. Western blotting was performed to detect the protein levels of TWIST and EMT-related genes. Luciferase reporter gene analysis and RNA immunoprecipitation (RIP) analysis confirmed the interaction between lncRNA SNHG3, miR-326, and TWIST. CCK-8 and Transwell assays were performed to detect cell proliferation, invasion, and migration abilities. The results showed that lncRNA SNHG3 and TWIST were highly expressed in GC cell lines, while miR-326 was expressed to a low degree. Moreover, lncRNA SNHG3 knockdown or miR-326 overexpression significantly inhibited cell proliferation, migration, and invasion of GC cell lines. In addition, TWIST overexpression can reverse the inhibition of lncRNA SNHG3 knockdown or miR-326 overexpression on cell proliferation, migration, and invasion. In conclusion, lncRNA SNHG3 may promote GC progression through the miR-326/TWIST axis, which may provide a new diagnostic and prognostic biomarker for GC.
长链非编码RNA小核仁RNA宿主基因3（SNHG3）在胃癌中的功能及可能机制尚未得到充分研究。本研究旨在探讨SNHG3在胃癌细胞系增殖、迁移和侵袭中的作用。采用RT-qPCR检测胃癌9811-P细胞系中SNHG3、miR-326和TWIST的表达。进行蛋白质免疫印迹法检测TWIST和上皮-间质转化（EMT）相关基因的蛋白水平。荧光素酶报告基因分析和RNA免疫沉淀（RIP）分析证实了长链非编码RNA SNHG3、miR-326和TWIST之间的相互作用。采用CCK-8和Transwell实验检测细胞增殖、侵袭和迁移能力。结果显示，长链非编码RNA SNHG3和TWIST在胃癌细胞系中高表达，而miR-326表达较低。此外，敲低长链非编码RNA SNHG3或过表达miR-326可显著抑制胃癌细胞系的增殖、迁移和侵袭。此外，过表达TWIST可逆转敲低长链非编码RNA SNHG3或过表达miR-326对细胞增殖、迁移和侵袭的抑制作用。综上所述，长链非编码RNA SNHG3可能通过miR-326/TWIST轴促进胃癌进展，这可能为胃癌提供一种新的诊断和预后生物标志物。
