长链非编码RNA NORAD通过靶向miR-202-5p促进甲状腺癌进展。
LncRNA NORAD promotes thyroid carcinoma progression through targeting miR-202-5p.
作者信息
He Hongjiang, Yang Hao, Liu Daming, Pei Rong
机构信息
Department of Head and Neck Surgery, Affiliated Tumor Hospital of Harbin Medical University No. 150, Haping Road, Nangang District, Harbin 150081, Heilongjiang, China.
出版信息
Am J Transl Res. 2019 Jan 15;11(1):290-299. eCollection 2019.
Abstract
Long noncoding RNAs (lncRNAs) have been shown to have several functional roles in tumor biology, and they are deregulated in many types of cancer. The role of a novel lncRNA, NORAD, in papillary thyroid carcinoma (PTC) is still unknown. In this study, we demonstrated that NORAD expression was upregulated in PTC cell lines and samples. Ectopic expression of NORAD promoted PTC cell growth, invasion and migration. Overexpression of NORAD promotes epithelial-to-mesenchymal transition (EMT) progression in the PTC cell. Furthermore, overexpression of NORAD suppressed miR-202-5p expression in PTC cells. The data suggested that miR-202-5p expression was downregulated in PTC cell lines and samples and was negatively correlated with NORAD expression in PTC tissues. Overexpression of miR-202-5p suppressed PTC cell growth, invasion and migration. In addition, we demonstrated that elevated expression of NORAD promoted PTC cell growth, invasion and migration by inhibiting miR-202-5p expression. These results suggested that the lncRNA NORAD acts as an oncogene in PTC progression, partly by regulating miR-202-5p expression.
摘要
长链非编码RNA（lncRNAs）已被证明在肿瘤生物学中具有多种功能作用，并且在多种癌症类型中其表达失调。一种新型lncRNA，即非编码RNA激活因子（NORAD），在甲状腺乳头状癌（PTC）中的作用仍然未知。在本研究中，我们证明NORAD在PTC细胞系和样本中表达上调。NORAD的异位表达促进了PTC细胞的生长、侵袭和迁移。NORAD的过表达促进了PTC细胞中的上皮-间质转化（EMT）进程。此外，NORAD的过表达抑制了PTC细胞中miR-202-5p的表达。数据表明，miR-202-5p在PTC细胞系和样本中的表达下调，并且在PTC组织中与NORAD表达呈负相关。miR-202-5p的过表达抑制了PTC细胞的生长、侵袭和迁移。此外，我们证明NORAD表达升高通过抑制miR-202-5p的表达促进了PTC细胞的生长、侵袭和迁移。这些结果表明lncRNA NORAD在PTC进展中作为一种癌基因发挥作用，部分是通过调节miR-202-5p的表达。
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