新鲜和冷冻的人类精子穿透去透明带仓鼠卵及牛宫颈黏液的情况。

Penetration of zona-free hamster ova and bovine cervical mucus by fresh and frozen human spermatozoa.

作者信息

Urry R L, Carrell D T, Hull D B, Middleton R G, Wiltbank M C

出版信息

Fertil Steril. 1983 May;39(5):690-4. doi: 10.1016/s0015-0282(16)47067-6.

Abstract

Semen samples from 25 fertile donors and 34 infertility patients were analyzed and evaluated both fresh and frozen with the zona-free hamster egg penetration test (EPT) and the bovine cervical mucus penetration test (MPT). Cryopreserved sperm penetrated 47.2% (average) of the hamster eggs, and fresh sperm penetrated 55.9%. Frozen sperm retained 86% of the fresh EPT values (P, not significant), but only 38% of the fresh MPT values (P less than 0.001). Cryopreserved sperm yielded the same fertility classification as fresh sperm in 94% of the cases with the EPT but only 43% with the MPT. The use of cryopreserved sperm simplifies the procedure and should enable more laboratories to utilize the EPT, but the poor results with the MPT preclude utilization of cryopreserved sperm for routine fertility evaluation with the MPT.

摘要

对来自25名生育能力正常的供者和34名不育患者的精液样本进行了分析和评估，分别采用无透明带仓鼠卵穿透试验（EPT）和牛宫颈黏液穿透试验（MPT）对新鲜样本和冷冻样本进行检测。冷冻保存的精子穿透了47.2%（平均）的仓鼠卵，新鲜精子穿透率为55.9%。冷冻精子保留了新鲜EPT值的86%（P，无显著性差异），但仅保留了新鲜MPT值的38%（P小于0.001）。在94%的EPT检测病例中，冷冻保存的精子与新鲜精子具有相同的生育能力分类，但在MPT检测中，这一比例仅为43%。冷冻保存精子的使用简化了操作过程，应该能使更多实验室采用EPT，但MPT检测结果不佳，妨碍了冷冻保存精子用于MPT常规生育能力评估。