Wilson A R, Cruickshank W J, Pugh-Humphreys R G, Jeffries A H
J Immunol Methods. 1978;19(2-3):205-16. doi: 10.1016/0022-1759(78)90180-1.
Mixtures of sheep erythrocytes and immune spleen cells from mice were incubated in shallow slide chambers coated with erythrocyte ghosts or anti-mouse Ig, with poly L-lysine as a coupling agent. Antigen-binding cells and erythrocytes surrounding antibody-releasing lymphocytes became bound to the reactive surfaces by immunocytoagglutination and could be readily observed on inversion of the chambers. The sensitivity of the method compares with those currently in use for quantification of antibody-releasing cells, and resolution of rosettes is markedly superior to that obtainable in other assay systems. The advantages and limitations of the method are discussed.
将绵羊红细胞与小鼠免疫脾细胞的混合物在涂有红细胞空壳或抗小鼠免疫球蛋白的浅玻片培养室中孵育，以聚L-赖氨酸作为偶联剂。抗原结合细胞以及围绕抗体释放淋巴细胞的红细胞通过免疫细胞凝集作用与反应表面结合，在培养室倒置时即可轻松观察到。该方法的灵敏度与目前用于定量抗体释放细胞的方法相当，并且玫瑰花结的分辨能力明显优于其他检测系统。本文讨论了该方法的优缺点。
