Eby W C, Chong C A, Dray S, Molinaro G A
J Immunol. 1975 Dec;115(6):1700-3.
Cells bearing immunoglobulin (Ig) can be enumerated by current methods, but cells secreting Ig (with unknown antibody specificity) can not. By using a reverse plaque-forming cell assay, we counted the Ig-secreting cells in the blood of nonimmunized individuals. Isolated human lymphocytes were plated in gels together with erythrocytes coated with purified antibody to human Ig. After treatment with anti-Ig antibody and complement, the Ig-secreting cells formed hemolytic plaques. In 10 normal individuals, 1.2 +/- 0.4% of the plated mononuclear cells formed plaques; in an individual tested on 7 different days during 3 months, 1.1 +/- 0.2% of the cells formed plaques. From the number of mononuclear cells in the blood, we calculated 22 +/- 8 Ig-secreting cells per mm3 of blood. Enumerating Ig-secreting cells is a new direct assay for a B cell function and need not correlate with quantitating serum Ig. Thus, this assay should be useful for studying Ig-secretion in normal and altered states.
携带免疫球蛋白（Ig）的细胞可用现有方法进行计数，但分泌Ig（抗体特异性未知）的细胞则无法计数。通过使用反向空斑形成细胞测定法，我们对未免疫个体血液中的Ig分泌细胞进行了计数。将分离的人淋巴细胞与包被有纯化抗人Ig抗体的红细胞一起铺在凝胶中。在用抗Ig抗体和补体处理后，Ig分泌细胞形成溶血空斑。在10名正常个体中，所铺板的单核细胞中有1.2±0.4%形成了空斑；在一名在3个月内7个不同日期接受检测的个体中，1.1±0.2%的细胞形成了空斑。根据血液中单核细胞的数量，我们计算出每立方毫米血液中有22±8个Ig分泌细胞。计数Ig分泌细胞是一种用于B细胞功能的新的直接测定法，无需与血清Ig定量相关。因此，该测定法对于研究正常状态和异常状态下的Ig分泌应是有用的。
