使用高速自适应光学眼底镜在人视网膜毛细血管中进行红细胞线密度的体内测量。
In vivo measurement of the lineal density of red blood cells in human retinal capillaries using high-speed adaptive optics ophthalmoscopy.
出版信息
Opt Lett. 2021 Jul 15;46(14):3392-3395. doi: 10.1364/OL.428538.
Abstract
We present an automated method for measuring the lineal density of red blood cells (RBCs) in human retinal capillaries using adaptive optics near-confocal ophthalmoscopy (AONCO). The spatiotemporal traces of RBCs flowing in retinal capillaries were extracted from AONCO images, enhanced using the Gabor filter, the vesselness filter, and the Hough transform. A total of 43 capillary segments (each 50 µm long) were analyzed in 12 eyes of 12 subjects, and the measurement error of the automated method was 0.09 cell over 50 µm compared with results obtained by manual counting. Our method provides a tool for assessing RBC spatial distribution in retinal capillaries.
摘要
我们提出了一种使用自适应光学近共焦检眼镜（AONCO）测量人视网膜毛细血管内红细胞（RBC）线密度的自动化方法。从 AONCO 图像中提取了在视网膜毛细血管中流动的 RBC 的时空轨迹，使用 Gabor 滤波器、血管滤波器和 Hough 变换进行增强。对 12 名受试者的 12 只眼中的 43 个毛细血管段（每个 50 µm 长）进行了分析，与手动计数相比，自动化方法的测量误差为 0.09 个细胞/50 µm。我们的方法为评估视网膜毛细血管内 RBC 的空间分布提供了一种工具。
相似文献
1
In vivo measurement of the lineal density of red blood cells in human retinal capillaries using high-speed adaptive optics ophthalmoscopy.使用高速自适应光学眼底镜在人视网膜毛细血管中进行红细胞线密度的体内测量。
Opt Lett. 2021 Jul 15;46(14):3392-3395. doi: 10.1364/OL.428538.
2
High-speed measurement of retinal arterial blood flow in the living human eye with adaptive optics ophthalmoscopy.自适应光学眼底镜高速测量活体人眼视网膜动脉血流。
Opt Lett. 2023 Apr 15;48(8):1994-1997. doi: 10.1364/OL.480896.
3
Adaptive optics ophthalmoscopy.自适应光学检眼镜检查法
J Refract Surg. 2000 Sep-Oct;16(5):S602-7. doi: 10.3928/1081-597X-20000901-23.
4
Noninvasive characterization of erythrocyte motion in human retinal capillaries using high-speed adaptive optics near-confocal imaging.使用高速自适应光学近共焦成像技术对人视网膜毛细血管中红细胞运动进行无创表征。
Biomed Opt Express. 2018 Jul 12;9(8):3653-3677. doi: 10.1364/BOE.9.003653. eCollection 2018 Aug 1.
5
Velocity measurements of normal and sickle red blood cells in the rat retinal and choroidal vasculatures.大鼠视网膜和脉络膜血管系统中正常和镰状红细胞的速度测量。
Microvasc Res. 2000 Nov;60(3):281-93. doi: 10.1006/mvre.2000.2270.
6
The source of moving particles in parafoveal capillaries detected by adaptive optics scanning laser ophthalmoscopy.自适应光学扫描激光检眼镜检测到的旁中心凹毛细血管内运动颗粒的来源。
Invest Ophthalmol Vis Sci. 2012 Jan 20;53(1):171-8. doi: 10.1167/iovs.11-8192.
7
Retinal hemorheologic characterization of early-stage diabetic retinopathy using adaptive optics scanning laser ophthalmoscopy.使用自适应光学扫描激光检眼镜对早期糖尿病视网膜病变进行视网膜血液流变学特征分析。
Invest Ophthalmol Vis Sci. 2014 Sep 11;55(12):8513-22. doi: 10.1167/iovs.14-15121.
8
In vivo imaging of microscopic structures in the rat retina.大鼠视网膜微观结构的体内成像。
Invest Ophthalmol Vis Sci. 2009 Dec;50(12):5872-9. doi: 10.1167/iovs.09-3675. Epub 2009 Jul 2.
9
Insights into Sickle Cell Disease through the Retinal Microvasculature: Adaptive Optics Scanning Light Ophthalmoscopy Correlates of Clinical OCT Angiography.通过视网膜微血管系统洞察镰状细胞病：自适应光学扫描激光检眼镜与临床光学相干断层扫描血管造影的相关性
Ophthalmol Sci. 2022 Jul 12;2(4):100196. doi: 10.1016/j.xops.2022.100196. eCollection 2022 Dec.
10
Measuring Temporal and Spatial Variability of Red Blood Cell Velocity in Human Retinal Vessels.测量人体视网膜血管中红细胞速度的时变和空间变异性。
Invest Ophthalmol Vis Sci. 2021 Nov 1;62(14):29. doi: 10.1167/iovs.62.14.29.
引用本文的文献
1
A High-Fidelity Computational Model for Predicting Blood Cell Trafficking and 3D Capillary Hemodynamics in Retinal Microvascular Networks.一种用于预测视网膜微血管网络中血细胞输运和 3D 毛细血管血液动力学的高保真计算模型。
Invest Ophthalmol Vis Sci. 2024 Nov 4;65(13):37. doi: 10.1167/iovs.65.13.37.
2
Changes to the shape, orientation and packing of red cells as a function of retinal capillary size.红细胞的形状、取向和堆积随视网膜毛细血管大小而变化。
Biomed Opt Express. 2024 Jan 3;15(2):558-578. doi: 10.1364/BOE.511093. eCollection 2024 Feb 1.
3
Differential reductions in the capillary red-blood-cell flux between retina and brain under chronic global hypoperfusion.慢性全身性低灌注情况下视网膜与脑之间毛细血管红细胞通量的差异降低。
Neurophotonics. 2023 Jul;10(3):035001. doi: 10.1117/1.NPh.10.3.035001. Epub 2023 Jun 14.
4
Evolution of adaptive optics retinal imaging [Invited].自适应光学视网膜成像的发展[特邀报告]
Biomed Opt Express. 2023 Feb 28;14(3):1307-1338. doi: 10.1364/BOE.485371. eCollection 2023 Mar 1.
本文引用的文献
1
Recovering the appearance of the capillary blood column from under-sampled flow data.从欠采样的流动数据中恢复毛细血管血柱的外观。
Opt Lett. 2020 Aug 1;45(15):4320-4323. doi: 10.1364/OL.398168.
2
Noninvasive characterization of erythrocyte motion in human retinal capillaries using high-speed adaptive optics near-confocal imaging.使用高速自适应光学近共焦成像技术对人视网膜毛细血管中红细胞运动进行无创表征。
Biomed Opt Express. 2018 Jul 12;9(8):3653-3677. doi: 10.1364/BOE.9.003653. eCollection 2018 Aug 1.
3
Adaptive optics imaging of the human retina.自适应光学视网膜成像。
Prog Retin Eye Res. 2019 Jan;68:1-30. doi: 10.1016/j.preteyeres.2018.08.002. Epub 2018 Aug 27.
4
High speed adaptive optics ophthalmoscopy with an anamorphic point spread function.具有变形点扩散函数的高速自适应光学检眼镜检查法。
Opt Express. 2018 May 28;26(11):14356-14374. doi: 10.1364/OE.26.014356.
5
En Face Doppler Optical Coherence Tomography Measurement of Total Retinal Blood Flow in Diabetic Retinopathy and Diabetic Macular Edema.糖尿病视网膜病变和糖尿病性黄斑水肿中视网膜总血流量的迎面多普勒光学相干断层扫描测量
JAMA Ophthalmol. 2017 Mar 1;135(3):244-251. doi: 10.1001/jamaophthalmol.2016.5774.
6
Measurements of Retinal Perfusion Using Laser Speckle Flowgraphy and Doppler Optical Coherence Tomography.使用激光散斑血流图和多普勒光学相干断层扫描测量视网膜灌注。
Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5417-5425. doi: 10.1167/iovs.16-19896.
7
Erythrocytes Are Oxygen-Sensing Regulators of the Cerebral Microcirculation.红细胞是脑微循环的氧传感调节因子。
Neuron. 2016 Aug 17;91(4):851-862. doi: 10.1016/j.neuron.2016.07.016. Epub 2016 Aug 4.
8
Enhancement of Vascular Structures in 3D and 2D Angiographic Images.三维和二维血管造影图像中的血管结构增强。
IEEE Trans Med Imaging. 2016 Sep;35(9):2107-2118. doi: 10.1109/TMI.2016.2550102. Epub 2016 Apr 4.
9
Adaptive optics ophthalmoscopy.自适应光学检眼镜检查法
Annu Rev Vis Sci. 2015 Nov;1:19-50. doi: 10.1146/annurev-vision-082114-035357. Epub 2015 Oct 14.
10
Doppler optical coherence tomography.多普勒光学相干断层扫描
Prog Retin Eye Res. 2014 Jul;41(100):26-43. doi: 10.1016/j.preteyeres.2014.03.004. Epub 2014 Apr 3.
Zotero 插件