Li Yongduo, Zhou Junlin
Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China.
Infect Drug Resist. 2021 Jul 8;14:2633-2644. doi: 10.2147/IDR.S315105. eCollection 2021.
This study aimed to construct drug-loading and drug-releasing quantitative equations for gentamicin sponges in addition to realizing a gentamicin sponge for wound infection prevention and treatment.
Sterile sponges were cut into pieces of 1×1 × 0.5 cm and immersed in 40, 16, 8, 4, 1.6, 0.8, or 0 mg/mL of gentamicin solution for 12, 24, 48, 96, or 120 h to evaluate their gentamicin loading. The sponges were subsequently immersed in the gentamicin solution of different concentrations for 48 h, air-dried, and then immersed in 10 mL of 0.9% physiological saline to evaluate the gentamicin release. Methicillin-sensitive (MSSA) and were used to explore the sponges' infection prevention scheme. In addition, a rat femur fracture with wound infection model was used to assess the infection treatment scheme.
The antibacterial zone sizes of the sponges immersed in 40, 16, 8, 4, 1.6, and 0.8 mg/mL of the gentamicin solution were larger than those of the 0 mg/mL air-dried sponge, and the difference was statistically significant (p < 0.01, p < 0.01, p < 0.01, p < 0.01, p < 0.01, and p < 0.01, respectively). The rats in the 40, 16, and 8 mg/mL air-dried sponge groups had no wound suppuration in either the MSSA or rat infection models.
A quantified equation for the sponges' gentamicin loading and release was achieved with high accuracy. Furthermore, we recommend the 40, 16, or 8 mg/mL air-dried sponge for the treatment of wounds with antibiotic-sensitive bacterial infections.
本研究旨在构建庆大霉素海绵的载药和释药定量方程，同时制备用于预防和治疗伤口感染的庆大霉素海绵。
将无菌海绵切成1×1×0.5 cm的小块，分别浸入40、16、8、4、1.6、0.8或0 mg/mL的庆大霉素溶液中12、24、48、96或120 h，以评估其庆大霉素载药量。随后将海绵浸入不同浓度的庆大霉素溶液中48 h，晾干，再浸入10 mL 0.9%生理盐水中以评估庆大霉素释放情况。使用对甲氧西林敏感的金黄色葡萄球菌（MSSA）来探索海绵的感染预防方案。此外，采用大鼠股骨骨折伴伤口感染模型评估感染治疗方案。
浸入40、16、8、4、1.6和0.8 mg/mL庆大霉素溶液的海绵的抑菌圈大小均大于晾干的0 mg/mL海绵，差异具有统计学意义（分别为p < 0.01、p < 0.01、p < 0.01、p < 0.01、p < 0.01和p < 0.01）。在MSSA或大鼠感染模型中，40、16和8 mg/mL晾干海绵组的大鼠伤口均未出现化脓。
成功建立了海绵庆大霉素载药和释药的高精度定量方程。此外，我们推荐使用40、16或8 mg/mL晾干海绵治疗对抗生素敏感细菌感染的伤口。
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