Husodo K, Kamal A F, Yusuf A A
Department of Orthopaedic and Traumatology, Faculty of Medicine Universitas Indonesia / Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Department of Histology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
J Orthop Surg (Hong Kong). 2016 Aug;24(2):245-9. doi: 10.1177/1602400224.
To evaluate the effect of povidone iodine and hydrogen peroxide on fracture healing in a rat model.
The middle section of the right femur of 36 male Sprague Dawley rats was osteotomised with a saw. In the control group (n=9), the wound was irrigated with 100 ml 0.9% saline. In the 10% povidone iodine (n=9), 1% povidone iodine (n=9), and 3% hydrogen peroxide (n=9) groups, the wound was completely soaked with the respective solution for 2 minutes, and then irrigated with 100 ml saline. The osteotomy was fixed with an intramedullary Kirschner wire. Rats were euthanised at week 1, 2, and 5. In each femur, the percentage area of osseous, cartilaginous, and fibrous tissue in the callus was evaluated in 3 slides (one median and 2 paramedian).
The control group differed significantly to the other 3 groups (p=0.023 to p<0.001) in weeks 1, 2, and 5, except for the 1% povidone iodine group in terms of percentage of osseous (p=0.349) and fibrous (p=0.999) tissue. The healing process was similar in the 1% povidone iodine group and the control group, whereas healing was impaired in the 10% povidone iodine group and 3% hydrogen peroxide group, as indicated by the lower percentage of osseous tissue, higher percentage of fibrous tissue, and increased percentage of cartilaginous tissue between weeks 2 and 5 (delayed bone healing).
The 1% povidone iodine solution is recommended as the irrigation adjuvant in fracture surgery.
在大鼠模型中评估聚维酮碘和过氧化氢对骨折愈合的影响。
用锯将36只雄性Sprague Dawley大鼠右股骨中段截断。对照组（n = 9）用100 ml 0.9%生理盐水冲洗伤口。在10%聚维酮碘组（n = 9）、1%聚维酮碘组（n = 9）和3%过氧化氢组（n = 9）中，伤口用相应溶液完全浸泡2分钟，然后用100 ml生理盐水冲洗。骨折处用髓内克氏针固定。在第1、2和5周对大鼠实施安乐死。在每根股骨中，在3张切片（1张正中切片和2张旁正中切片）上评估骨痂中骨组织、软骨组织和纤维组织的面积百分比。
在第1、2和5周，对照组与其他3组有显著差异（p = 0.023至p < 0.001），除了1%聚维酮碘组在骨组织（p = 0.349）和纤维组织（p = 0.999）百分比方面。1%聚维酮碘组和对照组的愈合过程相似，而10%聚维酮碘组和3%过氧化氢组的愈合受到损害，表现为在第2至5周骨组织百分比降低、纤维组织百分比升高以及软骨组织百分比增加（延迟骨愈合）。
推荐1%聚维酮碘溶液作为骨折手术中的冲洗佐剂。
