College of Animal Husbandry and Veterinary Medicine, Tibet Agricultural and Animal Husbandry University, Nyingchi, 860000, Tibet, People's Republic of China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Environ Sci Pollut Res Int. 2021 Dec;28(48):68288-68299. doi: 10.1007/s11356-021-15286-3. Epub 2021 Jul 16.
Tibial dyschondroplasia (TD) is a common skeletal disease occurred in growth plate of fast-growing broilers. Thiram is a sort of chemical used for pesticide and fungicide. The excessive use of thiram increased the threat to animal and human health. In this study, we aimed to investigate the therapeutic mechanism of chlorogenic acid (CGA) on thiram-induced tibial dyschondroplasia. Broiler chickens were divided into three different groups, e.g., control, TD, and CGA. CGA was administrated after the induction of TD from 4 day to 7 day. Biochemical analysis was performed to detect the content of calcium (Ca) and phosphorus (P). Histological changes and degradation of extracellular matrix were observed through hematoxylin-eosin (H & E) and Masson staining. To further determine the mechanism, TUNEL staining and western blot were also performed to detect the apoptosis changes in growth plate of all groups. The results showed the disproportionation of Ca and P content and upregulation of apoptosis during the development of TD. But, after the administration of CGA, the ratio of Ca:P was upregulated, and the apoptosis was also downregulated. The current study shows the toxic effect of thiram on chickens and suggests that CGA is associated with a mechanism that plays a significant role in apoptosis induced by thiram in poultry industry.
胫骨软骨发育不良（TD）是一种常见的骨骼疾病，发生在快速生长的肉鸡生长板中。福美双是一种用于农药和杀菌剂的化学物质。福美双的过度使用增加了对动物和人类健康的威胁。在这项研究中，我们旨在研究绿原酸（CGA）对福美双诱导的胫骨软骨发育不良的治疗机制。肉鸡被分为三组，即对照组、TD 组和 CGA 组。从第 4 天到第 7 天，在 TD 诱导后，CGA 被给予肉鸡。通过生化分析检测钙（Ca）和磷（P）的含量。通过苏木精-伊红（H＆E）和 Masson 染色观察细胞外基质的降解和组织学变化。为了进一步确定机制，还进行了 TUNEL 染色和 Western blot 以检测所有组生长板中的细胞凋亡变化。结果表明，在 TD 发育过程中 Ca 和 P 含量的失调和细胞凋亡的上调。但是，给予 CGA 后，Ca：P 比值上调，细胞凋亡也下调。本研究显示了福美双对鸡的毒性作用，并表明 CGA 与家禽业中由福美双诱导的细胞凋亡相关的机制有关。
