College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
College of Animals Husbandry and Veterinary Medicine, Tibet Agricultural and Animal Husbandry University, Linzhi, Tibet, 860000, People's Republic of China.
Environ Sci Pollut Res Int. 2019 Jun;26(16):16210-16219. doi: 10.1007/s11356-019-05032-1. Epub 2019 Apr 10.
Tibial dyschondroplasia (TD) is most the common tibiotarsal bone disease in rapidly growing birds throughout the world. There is accumulating evidence that COX-2 abnormal expression in tibia plays an important role in TD progression. So, the regulation of COX-2 is an ever more appealing target for therapeutic intervention in TD. Astragaloside IV has an indispensable role in maintaining COX-2 expression in many diseases. So, we designed this study to use Astragaloside IV (AST-IV) against TD-affected chickens. A total of 180 Arbor Acres chickens were randomly divided in the control group, TD group, and Astr (AST-IV-treated chickens) group. During the experiment, mortality, feed conversion ratio, physiological changes, biochemical criterion, liver antioxidant enzymes, and gene expression of COX-2 were examined in all the chicken groups at various days. The results showed that AST-IV administration restored the growth performance and tibia lesions and decreased the mortality as compared with TD chickens. The biochemical criterion (ALP, AST, and ALT) of serum and liver antioxidant enzymes (SOD, GSH-Px, MDA, and T-AOC) improved after the administration of AST-IV. The COX-2 gene was upregulated significantly (P < 0.05) in TD chickens. Whereas, AST-IV treatment downregulated both gene and protein expression of COX-2 significantly (P < 0.05) in TD-affected chickens. AST-IV recovered tibial dyschondroplasia chickens by increasing the growth performance, ameliorating tibial cartilage damage, and decreasing COX-2 expression. In conclusion, AST-IV can be used to prevent thiram-induced TD in chickens.
胫骨软骨发育不良（TD）是全世界快速生长鸟类中最常见的胫骨和跗跖骨疾病。有越来越多的证据表明，COX-2 在胫骨中的异常表达在 TD 进展中起着重要作用。因此，COX-2 的调节是 TD 治疗干预的一个更有吸引力的靶点。黄芪甲苷在许多疾病中对 COX-2 的表达起着不可或缺的作用。因此，我们设计了这项研究，用黄芪甲苷（AST-IV）来治疗患有 TD 的鸡。总共 180 只阿伯丁鸡被随机分为对照组、TD 组和 Astr（AST-IV 治疗鸡）组。在实验过程中，在不同的日子里，检查了所有鸡群的死亡率、饲料转化率、生理变化、生化标准、肝抗氧化酶和 COX-2 的基因表达。结果表明，与 TD 鸡相比，AST-IV 给药恢复了生长性能和胫骨病变，降低了死亡率。AST-IV 给药后，血清生化标准（ALP、AST 和 ALT）和肝抗氧化酶（SOD、GSH-Px、MDA 和 T-AOC）得到改善。COX-2 基因在 TD 鸡中显著上调（P<0.05）。然而，AST-IV 治疗显著下调了 TD 鸡中 COX-2 的基因和蛋白表达（P<0.05）。AST-IV 通过增加生长性能、改善胫骨软骨损伤和降低 COX-2 表达来恢复胫骨软骨发育不良鸡。总之，AST-IV 可用于预防 thiram 诱导的鸡胫骨软骨发育不良。
