Department of Thyroid Surgery, Affiliated Hospital of Southwest Medical University, Luzhou, China.
Department of Central Laboratory, Affiliated Hospital of Southwest Medical University, Luzhou, China.
J Clin Lab Anal. 2021 Sep;35(9):e23907. doi: 10.1002/jcla.23907. Epub 2021 Jul 16.
The miRNAs play critical roles in the progression of various tumors. Our study aimed to screen and identify miRNAs to investigate their diagnostic and prognostic value for papillary thyroid carcinoma (PTC).
miRNAs were evaluated in PTC (n = 30) tissues, A-PTC (n = 30), benign nodules (n = 35) and A-benign nodules (n = 35). The expression levels of five miRNAs were quantified using real-time, quantitative PCR. ROC analysis was used to evaluate the miRNA diagnostic value.
The expression of miR-1296-5p, miR-1301-3p, and miR-532-5p was significantly downregulated (p = 0.0001, p = 0.0006, p = 0.0024, respectively), while miR-551b-3p and miR-455-3p were significantly upregulated in PTC tissues compared to A-PTC tissues (p = 0.0005, p = 0.0046, respectively). Interestingly, the expression of miR-1296-5p was downregulated, while miR-551b-3p and miR-455-3p were upregulated in the A-PTC group compared to the A-benign group. Moreover, the miR-1296-5p expression level was associated with tumor size, the number of foci and the TNM stage; the miR-455-3p expression level was correlated with patient age, tumor size, and TNM stage; and the miR-532-5p expression level was correlated with patient age, lymph node metastasis and TNM stage correspondingly. ROC analysis revealed that the AUCs for miR-1301-3p, miR-1296-5p, miR-455-3p, miR-532-5p, and miR-551b-3p were 0.773, 0.790, 0.783, 0.744, and 0.650, respectively.
Our results indicated that miR-1296-5p, miR-1301-3p, miR-532-5p, miR-551b-3p, and miR-455-3p are aberrantly expressed in papillary thyroid carcinomas and correlated with clinicopathological features. ROC curve analysis indicated that these five miRNAs have a potential diagnostic value. Consequently, we speculate that the five altered miRNAs may serve as potential diagnostic and prognostic biomarkers for PTC.
miRNAs 在各种肿瘤的进展中发挥着关键作用。我们的研究旨在筛选和鉴定 miRNAs，以研究其对甲状腺乳头状癌（PTC）的诊断和预后价值。
评估了 30 例 PTC 组织、30 例 A-PTC、35 例良性结节和 35 例 A-良性结节中的 miRNAs 表达水平。使用实时定量 PCR 定量检测了 5 种 miRNA 的表达水平。ROC 分析用于评估 miRNA 的诊断价值。
miR-1296-5p、miR-1301-3p 和 miR-532-5p 的表达明显下调（p=0.0001、p=0.0006、p=0.0024），而 miR-551b-3p 和 miR-455-3p 在 PTC 组织中的表达明显上调与 A-PTC 组织相比（p=0.0005、p=0.0046）。有趣的是，miR-1296-5p 的表达在 A-PTC 组中下调，而 miR-551b-3p 和 miR-455-3p 在 A-良性组中上调。此外，miR-1296-5p 的表达水平与肿瘤大小、病灶数量和 TNM 分期有关；miR-455-3p 的表达水平与患者年龄、肿瘤大小和 TNM 分期相关；miR-532-5p 的表达水平与患者年龄、淋巴结转移和 TNM 分期相关。ROC 分析显示，miR-1301-3p、miR-1296-5p、miR-455-3p、miR-532-5p 和 miR-551b-3p 的 AUC 分别为 0.773、0.790、0.783、0.744 和 0.650。
我们的结果表明，miR-1296-5p、miR-1301-3p、miR-532-5p、miR-551b-3p 和 miR-455-3p 在甲状腺乳头状癌中表达异常，并与临床病理特征相关。ROC 曲线分析表明，这 5 个 miRNA 具有潜在的诊断价值。因此，我们推测这 5 个改变的 miRNA 可能作为 PTC 的潜在诊断和预后生物标志物。
