Department of Obstetrics and Gynecology, Hanchuan People's Hospital, Hanchuan, China.
Eur Rev Med Pharmacol Sci. 2019 Mar;23(6):2353-2359. doi: 10.26355/eurrev_201903_17379.
Ovarian cancer is one of the most ordinary malignant tumors. Recently, the role of long noncoding RNAs (lncRNAs) in tumor progression has caught attention of numerous researchers. In this research, lncRNA DARS-AS1 was studied to identify how it functions in the development of ovarian cancer.
DARS-AS1 expression was detected by Real-time quantitative polymerase chain reaction (RT-qPCR) in ovarian cancer tissue samples. Moreover, functional experiments were conducted to detect the effect of DARS-AS1 on the proliferation and metastasis of ovarian cancer. In addition, the underlying mechanism was explored through luciferase assay and RNA immunoprecipitation (RIP) assay.
In this study, DARS-AS1 expression was remarkably higher in ovarian cancer tissues compared with that in adjacent ones. Cell proliferation was inhibited after DARS-AS1 silenced in ovarian cancer cells. Moreover, cell migration and invasion were also inhibited after DARS-AS1 silenced in ovarian cancer cells. Furthermore, results of luciferase assays and RIP assay showed that microRNA-532-3p (miR-532-3p) was a direct target of DARS-AS1 in ovarian cancer. The expression of miR-532-3p was upregulated after DARS-AS1 was knocked down.
Our study suggests that DARS-AS1 enhances cell proliferation and metastasis via sponging miR-532-3p in ovarian cancer.
卵巢癌是最常见的恶性肿瘤之一。最近，长链非编码 RNA（lncRNA）在肿瘤进展中的作用引起了众多研究人员的关注。在这项研究中，研究了 lncRNA DARS-AS1，以确定其在卵巢癌发展中的作用。
通过实时定量聚合酶链反应（RT-qPCR）检测卵巢癌组织样本中的 DARS-AS1 表达。此外，进行了功能实验以检测 DARS-AS1 对卵巢癌细胞增殖和转移的影响。此外，通过荧光素酶测定和 RNA 免疫沉淀（RIP）测定探讨了潜在的机制。
在这项研究中，与相邻组织相比，卵巢癌组织中 DARS-AS1 的表达明显更高。沉默卵巢癌细胞中的 DARS-AS1 后，细胞增殖受到抑制。此外，沉默卵巢癌细胞中的 DARS-AS1 后，细胞迁移和侵袭也受到抑制。此外，荧光素酶测定和 RIP 测定的结果表明，miR-532-3p（miR-532-3p）是卵巢癌中 DARS-AS1 的直接靶标。敲低 DARS-AS1 后，miR-532-3p 的表达上调。
我们的研究表明，DARS-AS1 通过海绵吸附 miR-532-3p 增强卵巢癌细胞的增殖和转移。
