2nd Department of Neurosurgery, Xingtai People's Hospital, Xingtai, Hebei Province, China.
J BUON. 2021 May-Jun;26(3):984-991.
To explore the effect of LncRNA MALAT1 on the growth of glioma cells and its related mechanism.
The expression of LncRNA MALAT1 and its target gene miR-613 in glioma cells was detected by RT-PCR, and the proliferation, invasion and apoptosis rates and the expression of related proteins were detected by CCK-8, invasion and apoptosis rate tests.
The results of qRT-PCR showed that LncRNA MALAT1 was up-regulated and miR-613 was down-regulated in glioma tissues and cells, and LncRNA MALAT1 was negatively correlated with miR-613. Cell tests confirmed that LncRNA MALAT1 played a role in promoting oncogene, including promoting proliferation and invasion of glioma cells and promoting apoptosis. Bioinformatics prediction and subsequent experiments proved that miR-613 was the direct target of LncRNA MALAT1. Furthermore, the proliferation and invasion ability of glioma cells were evidently inhibited, the apoptosis rate was evidently enhanced, and the expressions of pro-apoptosis related proteins bax and Cle-Caspase-3 were up-regulated while the expression of anti-apoptosis related protein bcl-2 was down-regulated.
LncRNA MALAT1 can promote the proliferation and invasion of glioma cells and inhibit the apoptosis of glioma cells by sponging miR-613. LncRNA MALAT1 can be applied as a new potential target for glioma treatment.
探讨长链非编码 RNA MALAT1 对神经胶质瘤细胞生长的影响及其相关机制。
采用 RT-PCR 检测神经胶质瘤细胞中长链非编码 RNA MALAT1 及其靶基因 miR-613 的表达，采用 CCK-8 法、侵袭和凋亡率检测实验检测细胞增殖、侵袭和凋亡率及相关蛋白的表达。
qRT-PCR 结果显示，长链非编码 RNA MALAT1 在神经胶质瘤组织和细胞中呈上调表达，miR-613 呈下调表达，且长链非编码 RNA MALAT1 与 miR-613 呈负相关。细胞实验证实长链非编码 RNA MALAT1 发挥促癌基因作用，包括促进神经胶质瘤细胞的增殖和侵袭，促进细胞凋亡。生物信息学预测及后续实验证实 miR-613 是长链非编码 RNA MALAT1 的直接靶基因。进一步研究发现，长链非编码 RNA MALAT1 沉默后可显著抑制神经胶质瘤细胞的增殖和侵袭能力，促进细胞凋亡，上调促凋亡相关蛋白 bax 和 Cle-Caspase-3 的表达，下调抗凋亡相关蛋白 bcl-2 的表达。
长链非编码 RNA MALAT1 可通过海绵吸附 miR-613 促进神经胶质瘤细胞的增殖、侵袭，抑制细胞凋亡，有望成为神经胶质瘤治疗的新潜在靶点。
