Arica Y, Hasirci V N
Middle East Technical University, Department of Biological Sciences, Ankara, Turkey.
Biomaterials. 1987 Nov;8(6):489-95. doi: 10.1016/0142-9612(87)90087-1.
Glucose oxidase (GOD) was immobilized in a poly(2-hydroxyethyl methacrylate) (HEMA) membrane through matrix entrapment in order to investigate the effect of various parameters (e.g. concentration of ingredients, temperature, repeated interaction with glucose and shelf storage) on the activity of the enzyme. Permeability of the membrane to a model permeant was tested and SEMs were obtained. It was observed that upon immobilization the affinity of GOD towards glucose was substantially decreased, and increasing the GOD content of the membrane adversely affected the activity. Membranes with the highest enzyme activity were also found to be the most permeable. Changes were detected in the pH and temperature where GOD is most active. Membrane permeability was observed to increase when crosslinker, and/or HEMA concentrations were low. The same parameters were also found to alter the morphology of the membrane as observed under SEM.
通过基质包埋法将葡萄糖氧化酶（GOD）固定在聚甲基丙烯酸2-羟乙酯（HEMA）膜中，以研究各种参数（如成分浓度、温度、与葡萄糖的反复相互作用和货架储存）对该酶活性的影响。测试了膜对模型渗透物的渗透性，并获得了扫描电子显微镜图像。观察到固定化后GOD对葡萄糖的亲和力显著降低，且增加膜中GOD的含量会对活性产生不利影响。还发现具有最高酶活性的膜渗透性最强。在GOD活性最高的pH和温度条件下检测到了变化。当交联剂和/或HEMA浓度较低时，观察到膜的渗透性增加。在扫描电子显微镜下观察还发现，相同的参数也会改变膜的形态。
