Arica M Y, Hasirci V
Middle East Technical University, Department of Biological Sciences, Ankara, Turkey.
Biomaterials. 1993 Sep;14(11):809-16. doi: 10.1016/0142-9612(93)90002-j.
Glucose oxidase was entrapped between poly(2-hydroxyethyl methacrylate) membranes and conditions were optimized for high enzyme activity and high levels of entrapment. Highest entrapment was with a 78 microns thick coat. A continuous flow membrane reactor was designed and used. The reaction was first order with respect to glucose and to oxygen. Vmax values for the native and immobilized enzymes were 0.182 and 0.133 mM/min. The Km's for native and immobilized enzymes were 6.2 and 16.9 mM, respectively. At high substrate concentrations enzyme poisoning was detected. Both pH and temperature profiles moved to higher values upon immobilization. The enzyme retained 80% of its activity for at least 3 months in dry form.
葡萄糖氧化酶被包埋在聚（甲基丙烯酸2-羟乙酯）膜之间，并对条件进行了优化以获得高酶活性和高包埋水平。最高包埋率出现在78微米厚的涂层中。设计并使用了连续流膜反应器。该反应对葡萄糖和氧气呈一级反应。天然酶和固定化酶的Vmax值分别为0.182和0.133 mM/分钟。天然酶和固定化酶的Km值分别为6.2和16.9 mM。在高底物浓度下检测到酶中毒。固定化后，pH和温度曲线均向更高值移动。该酶以干燥形式至少可保留80%的活性达3个月。
