Department of Biological Sciences, University of Bergen, Norway.
Institute of Marine Research, Bergen, Norway.
Environ Toxicol Pharmacol. 2021 Oct;87:103704. doi: 10.1016/j.etap.2021.103704. Epub 2021 Jul 14.
A luciferase reporter gene-based bioassay battery consisting of stress-activated receptors from fish, complemented with traditional fish cell-based bioassays, were used to assess the toxicity of marine sediment samples from the Byfjorden area around the city of Bergen (Norway). The reporter assays covered a wide range of cellular signalling and metabolic pathways, representing different molecular initiating events in the adverse outcome pathway framework. Cytotoxicity, generation of reactive oxygen-species, and induction of 7-ethoxyresorufin-O-deethylase activity were analysed using fish liver and gill cell lines. Chemical analyses of the sediment extracts revealed complex contamination profiles, especially at the innermost stations, which contained a wide array of persistent organic pollutants, polycyclic aromatic hydrocarbons, and metals. Sediment extracts from these sites were more potent in activating the stress-activated receptors than the other extracts, reflecting their toxicant profiles. Importantly, receptor- and cell-based bioassays complemented the chemical analyses and provided important data for future environmental risk assessments of urban marine sediments.
采用基于荧光素酶报告基因的生物测定试剂盒，其中包含鱼类应激激活受体，同时辅以传统的鱼类细胞生物测定，对卑尔根市(Byfjorden)周边海域海洋沉积物样品的毒性进行了评估。该报告测定涵盖了广泛的细胞信号和代谢途径，代表了不良结局途径框架中的不同分子起始事件。使用鱼肝和鳃细胞系分析了细胞毒性、活性氧生成和 7-乙氧基resorufin-O-脱乙基酶活性的诱导。对沉积物提取物的化学分析揭示了复杂的污染状况，尤其是在内陆站点，这些站点含有多种持久性有机污染物、多环芳烃和金属。这些位点的沉积物提取物比其他提取物更能激活应激激活受体，反映了它们的毒性特征。重要的是，受体和基于细胞的生物测定补充了化学分析，并为未来城市海洋沉积物的环境风险评估提供了重要数据。
