School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China.
School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Key Laboratory of Anaerobic Biotechnology, Wuxi 214122, China; Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material, Suzhou 215011, China.
Bioresour Technol. 2021 Oct;338:125493. doi: 10.1016/j.biortech.2021.125493. Epub 2021 Jul 4.
Carbon materials are promising in improving the performance of anaerobic digestion, however, interactive mechanisms between the carbon-based enhancement and operating parameters remained unclear. Using anaerobic digested sludge as inoculum, the effects of Taihu blue algae biochar (ABC) on methanogenesis at different inoculation ratios were investigated during sludge anaerobic digestion. Results showed that ABC enhanced methane productions at the lower inoculation ratios (4% and 1%, v/v), but not at the higher ratio (10%, v/v). Mechanism analysis demonstrated methanogenic improvements at the lower inoculation ratios were not owing to initial organic loading rate increments. Otherwise, ABC addition at the lower inoculation ratios were more favorable for the enrichment of Methanosarcina than the higher ratio, which might be benefit for methanogenesis through directed interspecies electron transfer. Thus, for the improvement of sludge anaerobic digestion, the microbial enrichments at different inoculation ratios would be more important than the merely biochar addition.
碳材料在改善厌氧消化性能方面具有很大的潜力，但碳基增强材料与操作参数之间的相互作用机制仍不清楚。本研究采用厌氧消化污泥作为接种物，考察了太湖蓝藻生物炭（ABC）在不同接种比例下对污泥厌氧消化产甲烷的影响。结果表明，ABC 在较低的接种比例（4%和 1%，v/v）下增强了甲烷的产生，但在较高的接种比例（10%，v/v）下则没有。机理分析表明，较低接种比例下甲烷生成的改善不是由于初始有机负荷率的增加。相反，在较低的接种比例下添加 ABC 更有利于富集产甲烷菌，而不是较高的接种比例，这可能有利于通过定向种间电子转移进行甲烷生成。因此，对于污泥厌氧消化的改善，不同接种比例下的微生物富集比单纯添加生物炭更为重要。
