School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan.
School of Agriculture, Meiji University, Kawasaki, Kanagawa 214-8571, Japan.
Arch Biochem Biophys. 2021 Oct 15;710:108988. doi: 10.1016/j.abb.2021.108988. Epub 2021 Jul 16.
Monocytes are differentiated into macrophages. In this study, mitochondrial DNA copy number (mtDNAcn) levels and downstream events such as the expression of respiratory chain mRNAs were investigated during the phorbol 12-myristate 13-acetate (PMA)-induced differentiation of monocytes. Although PMA treatment increased mtDNAcn, the expression levels of mRNAs encoded in mtDNA were decreased. The levels of mitochondrial transcription factor A mRNA and protein were also decreased. The levels of coenzyme Q10 remained unchanged. These results imply that, although mtDNAcn is considered as a health marker, the levels of mtDNAcn may not always be consistent with the parameters of mitochondrial functions.
单核细胞分化为巨噬细胞。在这项研究中，研究了佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）诱导单核细胞分化过程中线粒体 DNA 拷贝数（mtDNAcn）水平和下游事件，如呼吸链 mRNAs 的表达。尽管 PMA 处理增加了 mtDNAcn，但线粒体 DNA 编码的 mRNAs 的表达水平下降。线粒体转录因子 A mRNA 和蛋白质的水平也下降。辅酶 Q10 的水平保持不变。这些结果表明，尽管 mtDNAcn 被认为是健康标志物，但 mtDNAcn 的水平并不总是与线粒体功能的参数一致。
