Okamoto Mizuho, Nakamura Akari, Maeda Ayaka, Kameda Yuka, Sugawara Kyosuke, Takekawa Marisa, Ogino Momoko, Makino Eriko, Ishii Nagisa, Fujisawa Akio, Yamamoto Yorihiro, Kashiba Misato
School of Bionics, Tokyo University of Technology, 1404-1 Katakura-machi, Hachioji, Tokyo 192-0982, Japan.
J Clin Biochem Nutr. 2022 May;70(3):231-239. doi: 10.3164/jcbn.21-148. Epub 2022 Feb 8.
Coenzyme Q10 is an important molecule for mitochondrial respiration and as an antioxidant. Maintenance of the ovum in a good condition is considered to be important for successful fertilization and development, which has been reported to be promoted by coenzyme Q10. In this study, we investigated the level of coenzyme Q10 during ovum fertilization and maturation. We attempted to analyze coenzyme Q10 levels during ovum development in species that use coenzyme Q10 but not coenzyme Q9. It was shown that medaka produces coenzyme Q10. We then measured the amount of coenzyme Q10 after fertilization of medaka ovum and found that it increased. The amount of free cholesterol biosynthesized from acetyl CoA as well as coenzyme Q10 increased during development, but the increase in coenzyme Q10 was more pronounced. The mRNA expression level of coq9 also increased during embryonic development, but the mRNA expression levels of other coenzyme Q10 synthases did not. These results suggest that the coq9 gene is upregulated during the development of medaka ovum after fertilization, resulting in an increase in the amount of coenzyme Q10 in the ovum. Medaka, which like humans has coenzyme Q10, is expected to become a model animal for coenzyme Q10 research.
辅酶Q10是线粒体呼吸的重要分子，也是一种抗氧化剂。维持卵子的良好状态被认为对成功受精和发育很重要，据报道辅酶Q10可促进这一过程。在本研究中，我们调查了卵子受精和成熟过程中辅酶Q10的水平。我们试图分析在使用辅酶Q10而非辅酶Q9的物种的卵子发育过程中辅酶Q10的水平。结果表明，青鳉会产生辅酶Q10。然后我们测量了青鳉卵子受精后辅酶Q10的含量，发现其有所增加。在发育过程中，由乙酰辅酶A生物合成的游离胆固醇以及辅酶Q10的含量均增加，但辅酶Q10的增加更为明显。coq9的mRNA表达水平在胚胎发育过程中也有所增加，但其他辅酶Q10合成酶的mRNA表达水平没有变化。这些结果表明，受精后青鳉卵子发育过程中coq9基因上调，导致卵子中辅酶Q10含量增加。与人类一样拥有辅酶Q10的青鳉有望成为辅酶Q10研究的模式动物。
