School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
Bioresour Technol. 2021 Oct;338:125546. doi: 10.1016/j.biortech.2021.125546. Epub 2021 Jul 13.
The aim of this study was to use metagenomics to investigate how Illite/smectite clay (I/S) affected Auxiliary Activities (AA1, AA2, AA3) thereby enhancing lignin decomposition and humification. Metagenomics analysis illustrated that the abundances of AA1, AA2, AA3 in test group (TG) with 10% I/S were 28.98%, 15.18%, 14.36% higher than that in reference group (RG), respectively. Meanwhile, I/S greatly boosted the efficiency of lignin degradation (17.96%) and humus formation (7.16%) compared with RG (13.10%, 3.49%). Furthermore, Actinobacteria was the microorganism with the greatest contribution in RG and TG to secreting AA1 (41.12%, 57.37%), AA2 (62.42%, 65.28%), AA3 (47.04%, 55.47%). Redundancy analysis (RDA) demonstrated that I/S could make the laccase encoding gene-AA1 contribute more to HS formation relative to AA2 and AA3. In conclusion, applying I/S in cattle manure composting effectively improved the abundance, bioavailability of lignin degradation functional gene enzymes and the composting efficiency.
本研究旨在利用宏基因组学来研究伊利石/蒙脱石粘土 (I/S) 如何影响辅助活性 (AA1、AA2、AA3)，从而增强木质素分解和腐殖化。宏基因组学分析表明，添加 10% I/S 的实验组 (TG) 中 AA1、AA2、AA3 的丰度分别比对照组 (RG) 高 28.98%、15.18%、14.36%。同时，与 RG (13.10%、3.49%)相比，I/S 大大提高了木质素降解 (17.96%)和腐殖质形成 (7.16%)的效率。此外，放线菌是 RG 和 TG 中分泌 AA1 (41.12%、57.37%)、AA2 (62.42%、65.28%)、AA3 (47.04%、55.47%)的微生物，贡献最大。冗余分析 (RDA) 表明，I/S 可以使漆酶编码基因-AA1 相对于 AA2 和 AA3 更多地促进 HS 的形成。总之，在牛粪堆肥中添加 I/S 可以有效提高木质素降解功能基因酶的丰度、生物可利用性和堆肥效率。
